Generic Caspase Activity Assay Kit (Fluorometric - Green) (ab112130)

製品の概要

  • 製品名Generic Caspase Activity Assay Kit (Fluorometric - Green)
    Caspase キット 製品一覧
  • 検出方法Fluorescent
  • サンプルの種類
    Adherent cells, Suspension cells
  • アッセイタイプDirect
  • 種交差性
    交差種: Human
  • 製品の概要

    Abcam's Activity Assay kits are a set of tools for monitoring cellular functions. The activation of caspase is widely accepted as a reliable indicator for cell apoptosis. Most caspases have substrate selectivity for the peptide sequence Val-Ala-Asp (VAD). ab112130 uses TF2-VAD-FMK as a fluorescent indicator for most caspase activities. The cell permeable and nontoxic TF2-VAD-FMK irreversibly binds to activated caspase-1, -3, -4, -5, -6, -7, -8 and -9 in apoptotic cells. Once bound to caspases, the fluorescent reagent is retained inside the cell. The binding event prevents the caspases from further catalysis but will not stop apoptosis from proceeding. Within 15 minutes incubation, it starts to react with active caspase enzymes.

    ab112130 provides all the essential components with an optimized assay protocol. It is designed to detect cell apoptosis by measuring generic activation of caspases (caspase-1, -3, -4, -5, -6, -7, -8 and -9) in live cells.

    ab112130 is used for either the quantification of most activated caspase activities in apoptotic cells or screening of caspase inhibitors. TF2-VAD-FMK, the green label reagent, allows for direct detection of activated caspases in apoptotic cells by a flow cytometer at Ex/Em = 488/520 nm.

    Visit our FAQs page for tips and troubleshooting.

  • 特記事項

    ab112130 should be stored dessicated.

  • アプリケーション適用あり: Flow Cyt, Functional Studiesmore details
  • 試験プラットフォームFlow

製品の特性

  • 関連性Caspases are members of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce 2 subunits, large and small, that dimerize to form the active enzyme.
  • 細胞内局在Cytoplasmic

アプリケーション

Our Abpromise guarantee covers the use of ab112130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration.
Functional Studies Use at an assay dependent concentration.

Generic Caspase Activity Assay Kit (Fluorometric - Green) 画像

  • The increase in TF2-VAD-FMK (Component A) fluorescence intensity with the addition of Camptothecin in Jurkat cells.
    Jurkat cells were untreated (Blue) or with 20 μM camptothecin (Pink) in a 37 °C, 5 % CO2 incubator for 4-5 hours, and then dye loaded with TF2-VAD-FMK for 1 hour.

プロトコール

Generic Caspase Activity Assay Kit (Fluorometric - Green) (ab112130) 使用論文

ab112130 has not yet been referenced specifically in any publications.

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Thank you for your reply.

You are correct that you can your cells for up to 24hours at 4C after they have been fixed. After the cells have been fixed. The kit is intended to be used on live cells.

Please let me know if there is anyt...

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Thank you forcontacting Abcam.

Youcan store your samples at 4C for up to 24hours, but you cannot freeze them.

Please let me know if there is anything else I can help you with.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"