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Recombinant fragment, corresponding to amino acids 110-209 of Human Geminin (AAH05185) with a proprietary tag.
Our Abpromise guarantee covers the use of ab104306 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration. Detection limit for recombinant tagged Geminin is approximately 0.03ng/ml when used as a capture antibody.|
|IHC-P||Use a concentration of 1 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|ICC/IF||Use a concentration of 10 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 24 kDa.|
|Flow Cyt||Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.|