製品の概要

  • 製品名Anti-Gemin 5 antibody
    Gemin 5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Gemin 5
  • アプリケーション適用あり: WB, IPmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Chimpanzee
  • 免疫原

    A region within amino acids 1360-1410 of Human Gemin 5 (NP_056280.1)

  • ポジティブ・コントロール
    • HeLa whole cell lysate. 293T cells.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab99360 was affinity purified using an epitope specific to Gemin 5 immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab99360 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 169 kDa.
IP Use at 2-5 µg/mg of lysate.

ターゲット情報

  • 関連性Gemin 5 is part of a large macromolecular complex localized to both the cytoplasm and the nucleus that plays a role in the cytoplasmic assembly of small nuclear ribonucleoproteins (snRNPs). Other members of this complex include SMN, Gemin 2 (SIP1), Gemin 3 (DDX20), and Gemin 4.
  • 細胞内局在Nuclear and cytoplasmic
  • 参照データベース
  • 別名
    • gem (nuclear organelle) associated protein 5 antibody
    • Gem-associated protein 5 antibody
    • GEMIN5 antibody

Anti-Gemin 5 antibody 画像

  • All lanes : Anti-Gemin 5 antibody (ab99360) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cells at 50 µg

    Developed using the ECL technique

    Predicted band size : 169 kDa


    Exposure time : 10 seconds
  • ab99360 at 1 µg/ml detecting Gemin 5 in HeLa whole cell lysate by western blot analysis following immunoprecipitation. Detection utilised ECL with a 1 second exposure.
    For immunoprecipitation, ab99360 was used at at 3 µg/mg lysate; 1 mg of lysate was used for IP and 20% of IP was loaded.
    Lane 1; IP using ab99360
    Lane 2; IP using control IgG.

Anti-Gemin 5 antibody (ab99360) 使用論文

ab99360 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"