Rabbit polyclonal to Gemin 4
Chimpanzee, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residue 1010 and the C-terminus (residue 1058) of Human Gemin 4 (NP_056536.1).
Whole cell lysate from HeLa and 293T cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
1/2000 - 1/10000. Detects a band of approximately 117 kDa (predicted molecular weight: 120 kDa).
Use at 2-5 µg/mg of lysate.
GEMIN 4 is part of the SMN complex and plays an essential role in spliceosomal snRNP assembly in the cytoplasm. It is required for pre mRNA splicing in the nucleus.
Cytoplasmic and nuclear.
Component of gems 4 antibody
Gem associated protein 4 antibody
HHRF 1 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 4 antibody (ab70750)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Gemin 4 with ab70750 at 1/200 (1µg/ml). Detection: DAB.
Western blot - Gemin 4 antibody (ab70750)
All lanes :
Anti-Gemin 4 antibody (ab70750) at 1/5000 dilution
Lane 1 :
HeLa whole cell lysate at 50 µg
Lane 2 :
HeLa whole cell lysate at 15 µg
Lane 3 :
HeLa whole cell lysate at 5 µg
Lane 4 :
293T whole cell lysate at 50 µg
Developed using the ECL technique
Predicted band size :
Observed band size :
117 kDa (
why is the actual band size different from the predicted?
Additional bands at :
160 kDa,270 kDa. We are unsure as to the identity of these extra bands.
Exposure time :
Immunoprecipitation - Gemin 4 antibody (ab70750)
Detection of Gemin 4 by Western Blot of Immunprecipitate.
ab70750 at 1µg/ml staining Gemin 4 in HeLa whole cell lysate immunoprecipitated using ab70750 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: chemiluminescence with exposure time of 30 seconds.
has not yet been referenced specifically in any publications.
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