Rabbit polyclonal to Gemin 4
Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human Gemin 4.
(Peptide available as
ab31581 gave a positive result in the following whole cell lysates:
HeLa (Human epithelial carcinoma cell line),
Jurkat (Human T cell lymphoblast-like cell line),
A431 (Human epithelial carcinoma cell line),
HEK 293 (Human embryonic kidney cell line),
MCF-7 (Human breast adenocarcinoma cell line),
SHSY-5Y (Human neuroblastoma cell line).
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
Immunogen affinity purified
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Detects a band of approximately 110 kDa (predicted molecular weight: 120 kDa).
Use a concentration of 5 µg/ml.
GEMIN 4 is part of the SMN complex and plays an essential role in spliceosomal snRNP assembly in the cytoplasm. It is required for pre mRNA splicing in the nucleus.
Cytoplasmic and nuclear.
Component of gems 4 antibody
Gem associated protein 4 antibody
HHRF 1 antibody
Western blot - Anti-Gemin 4 antibody (ab31581)
All lanes :
Anti-Gemin 4 antibody (ab31581) at 1 µg/ml
Lane 1 :
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat whole cell lysate ( ab7899) Lane 3 : A431 whole cell lysate ( ab7909) Lane 4 : HEK293 whole cell lysate ( ab7902) Lysates/proteins at 10 µg per lane. Secondary IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution Performed under reducing conditions. Predicted band size : 120 kDa Observed band size : 110 kDa ( why is the actual band size different from the predicted?)
Immunocytochemistry/ Immunofluorescence - Anti-Gemin 4 antibody (ab31581)
ICC/IF image of ab31581 stained human HeLa cells. The cells were PFA fixed (10 min), permabilised in TBS-T (20 min) and incubated with the antibody (ab31581, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to quench autofluorescence and block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).
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