製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Gemin 3
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • 免疫原

    Synthetic peptide corresponding to a region between residues 600 and 650 of human Gemin 3 (NP_009135.3).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab70896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 92 kDa).
IP Use at 1-4 µg/mg of lysate.
IHC-P 1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. It may also play a role in the metabolism of snoRNPs.
  • 組織特異性
    Ubiquitous.
  • 配列類似性
    Belongs to the DEAD box helicase family. DDX20 subfamily.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • 細胞内局在
    Cytoplasm. Nucleus > gem. Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Component of gems 3 antibody
    • DDX 20 antibody
    • ddx20 antibody
    • DDX20_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 20 antibody
    • DEAD box protein 20 antibody
    • DEAD box protein DP 103 antibody
    • DEAD box protein DP103 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 103kD antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide 20 antibody
    • DEAD/H BOX 20 antibody
    • DKFZP434H052 antibody
    • DP 103 antibody
    • DP103 antibody
    • Gemin-3 antibody
    • Gemin3 antibody
    • Probable ATP dependent RNA helicase DDX20 antibody
    • Probable ATP-dependent RNA helicase ddx20 antibody
    • SMN interacting protein antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Gemin 3 with ab70896 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-Gemin 3 antibody (ab70896) at 0.04 µg/ml

    Lane 1 : Whole cell lysate from HeLa cells at 50 µg
    Lane 2 : Whole cell lysate from HeLa cells at 15 µg
    Lane 3 : Whole cell lysate from HeLa cells at 5 µg

    Developed using the ECL technique

    Predicted band size : 92 kDa
    Observed band size : 110 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 120 kDa,175 kDa,58 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Detection of Human Gemin 3 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP), using ab70896 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.

参考文献

ab70896 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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