Rabbit polyclonal to Gemin 3
Horse, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to a region between residues 600 and 650 of human Gemin 3 (NP_009135.3).
- Whole cell lysate from HeLa cells.
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
Immunogen affinity purified
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
||1/1000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 92 kDa).
||Use at 1-4 µg/mg of lysate.
||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
The SMN complex plays an essential role in spliceosomal snRNP assembly in the cytoplasm and is required for pre-mRNA splicing in the nucleus. It may also play a role in the metabolism of snoRNPs.
Belongs to the DEAD box helicase family. DDX20 subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Cytoplasm. Nucleus > gem. Localized in subnuclear structures next to coiled bodies, called Gemini of Cajal bodies.
Information by UniProt
- Component of gems 3 antibody
- DDX 20 antibody
- ddx20 antibody
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Gemin 3 antibody (ab70896)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labelling Gemin 3 with ab70896 at 1/200 (1µg/ml). Detection: DAB.
Western blot - Gemin 3 antibody (ab70896)
All lanes :
Anti-Gemin 3 antibody (ab70896) at 0.04 µg/mlLane 1 :
Whole cell lysate from HeLa cells at 50 µgLane 2 :
Whole cell lysate from HeLa cells at 15 µgLane 3 :
Whole cell lysate from HeLa cells at 5 µg
Developed using the ECL techniquePredicted band size :
92 kDaObserved band size :
110 kDa (why is the actual band size different from the predicted?
)Additional bands at :
120 kDa,175 kDa,58 kDa. We are unsure as to the identity of these extra bands.Exposure time :
Immunoprecipitation - Gemin 3 antibody (ab70896)
Detection of Human Gemin 3 by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP), using ab70896 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection.
has not yet been referenced specifically in any publications.
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