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RabMAb

Anti-Gelsolin plasma 抗体 [EPR1941Y] (ab75832)

製品の概要

  • 製品名
    Anti-Gelsolin plasma antibody [EPR1941Y]
    Gelsolin plasma 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1941Y] to Gelsolin plasma
  • 特異性
    The antibody only detects the isoform 1 of the protein (86kDa).
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide within Human Gelsolin plasma aa 1-100 (N terminal). The exact sequence is proprietary.
    Database link: P06396-1

  • ポジティブ・コントロール
    • Human plasma
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab75832 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/25000 - 1/50000. Detects a band of approximately 90 kDa (predicted molecular weight: 86 kDa).
IP 1/50.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for Flow Cyt or ICC.
  • ターゲット情報

    • 関連性
      Gelsolin is a calcium regulated, actin modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. It plays a role in phosphoinositide mediated disassembly of actin filaments in Sertoli cell adhesion complexes; it may regulate sperm release and turnover of the blood testis barrier [RGD], and severs actin filaments in the presence of submicromolar calcium, thereby solating cytoplasmic actin gels. A calcium independent mechanism reverses the process. Northern blot analysis suggested that a single gene encodes both cell and plasma gelsolins. This protein may be unique in that it is made for both secretion and intracytoplasmic location.
    • 細胞内局在
      Secreted
    • 参照データベース
    • 別名
      • Actin depolymerizing factor antibody
      • ADF antibody
      • AGEL antibody
      • Brevin antibody
      • Gelsolin antibody
      see all

    画像

    • Lanes 1 - 4 : Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832) at 1/10000 dilution
      Lanes 5 - 8 : Anti-Gelsolin antibody [EPR1942] (ab109014) at 1/20000 dilution
      Lanes 9 - 12 : Anti-Gelsolin antibody [EP1940Y] (ab134183) at 1/10000 dilution

      Lane 1 : THP-1 (human acute monocytic leukemia) whole cell lysate
      Lane 2 : THP-1 cultured medium
      Lane 3 : MCF-7 (human breast adenocarcinoma) whole cell lysate
      Lane 4 : Human Plasma
      Lane 5 : THP-1 (human acute monocytic leukemia) whole cell lysate
      Lane 6 : THP-1 cultured medium
      Lane 7 : MCF-7 (human breast adenocarcinoma) whole cell lysate
      Lane 8 : Human Plasma
      Lane 9 : THP-1 (human acute monocytic leukemia) whole cell lysate
      Lane 10 : THP-1 cultured medium
      Lane 11 : MCF-7 (human breast adenocarcinoma) whole cell lysate
      Lane 12 : Human Plasma

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 86 kDa
      Observed band size : 81/86 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 seconds

      Bloking buffer 5% NFDM/TBST
      Diluting buffer 5% NFDM/TBST

      The secretory isoform of gelsolin is at 86kDa. The cytoplasmic isoforms of gelsolin are at 81kDa. Lane 5-8: ab109014 detected both secretory and cytoplasmic isoforms. Lane 9-12: ab134183 detected both secretory and cytoplasmic isoforms.

    • Anti-Gelsolin plasma antibody [EPR1941Y] (ab75832) at 1/50000 dilution + human plasma at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 86 kDa
      Observed band size : 90 kDa (why is the actual band size different from the predicted?)
    • ab75832 Immunoprecipitating Gelsolin plasma in mouse embryo whole cell lysate. 2µg of capture antibody (ab75832) per 0.35mg of lysate was used. 10µg of plasma was incubated with primary antibody (Panel A: ab75832, Panel B: ab134183) at a dilution of 1/1000 and VeriBlot for IP secondary antibody (HRP) ab131366 was used as the secondary antibody at a dilution of 1/10000.

      Lane 1: Human plasma
      Lane 2: Human plasma
      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab75832 in Human plasma.

      Exposure time: Panel A - 8 seconds, Panel B - 3 minutes.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      The additional band at 50 kDa is a rabbit IgG heavy chain.

    • ab75832 at 1/100 dilution staining Gelsolin plasma in human colon tissue, using a HRP/AP polymerized secondary antibody.

    参考文献

    This product has been referenced in:
    • Hashimoto K  et al. The potential of neurotensin secreted from neuroendocrine tumor cells to promote gelsolin-mediated invasiveness of prostate adenocarcinoma cells. Lab Invest 95:283-95 (2015). Read more (PubMed: 25581609) »
    • Polioudakis D  et al. MiR-191 Regulates Primary Human Fibroblast Proliferation and Directly Targets Multiple Oncogenes. PLoS One 10:e0126535 (2015). WB . Read more (PubMed: 25992613) »

    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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