製品の概要

  • 製品名Anti-Gelsolin antibody [GS-2C4]
    Gelsolin 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [GS-2C4] to Gelsolin
  • アプリケーション適用あり: Other, ELISA, WB, ICC/IF, IHC-Frmore details
  • 種交差性
    交差種: Sheep, Rabbit, Cow, Human, Pig
  • 免疫原

    Full length native protein (purified) (Human).

  • エピトープThe antibody is directed against an epitope located on the 47 kD peptide derived from a chymotryptic cleavage of gelsolin. This peptide contains the carboxy terminal actin binding site for gelsolin.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab11081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Other Use at an assay dependent concentration.
ELISA 1/1000.
AP Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 86 kDa.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
  • 組織特異性Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
  • 関連疾患Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
  • 配列類似性Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
  • 翻訳後修飾Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
  • 細胞内局在Cytoplasm > cytoskeleton and Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Actin depolymerizing factor antibody
    • Actin-depolymerizing factor antibody
    • ADF antibody
    • AGEL antibody
    • Brevin antibody
    • DKFZp313L0718 antibody
    • GELS_HUMAN antibody
    • Gelsolin antibody
    • Gsn antibody
    see all

Anti-Gelsolin antibody [GS-2C4] 画像

  • Anti-Gelsolin antibody [GS-2C4] (ab11081) at 1/2000 dilution + Human Plasma

    Secondary
    AP conjugated anti-mouse Fab IgG

    Performed under reducing conditions.

    Predicted band size : 86 kDa
  • ICC/IF image of ab11081 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab11081, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.

Anti-Gelsolin antibody [GS-2C4] (ab11081) 使用論文

This product has been referenced in:
  • Ostler N  et al. Gamma interferon-induced guanylate binding protein 1 is a novel actin cytoskeleton remodeling factor. Mol Cell Biol 34:196-209 (2014). Read more (PubMed: 24190970) »
  • Hibbert B  et al. Glycogen synthase kinase-3ß inhibition augments diabetic endothelial progenitor cell abundance and functionality via cathepsin B: a novel therapeutic opportunity for arterial repair. Diabetes 63:1410-21 (2014). Human . Read more (PubMed: 24296714) »

See all 13 Publications for this product

Product Wall

Application Western blot
Loading amount 10 µg
Gel Running Conditions Reduced Denaturing (8-16%)
Sample Sheep Serum (Cervical mucus)
Specification Cervical mucus
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Username

Mr. Guillaume TSIKIS

Verified customer

投稿 Jul 05 2013

Thank you for your inquiry and please excuse the delayed answer. I was in contact with the source of this antibody and did a literature search, but I could not find any other anti-gelsolin antibodies that were used with ab11081 as part of a sandwich...

Read More

Thank you for contacting us. The immunogen is the full-length human gelsolin, and the epitope that it binds to is located somewhere on the carboxyl-terminal two-thirds. The following reference describes production of monoclonal antibodies against human...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (glioblastoma cell lines)
Loading amount 8 µg
Specification glioblastoma cell lines
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Nov 22 2006

The products, as long as you follow the storage instructions can be kept up to 5years,you should aliquot them and store them at -20°C and avoid freeze / thaw cycles. If you need any further information, please don't hestitate to contact us.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application ELISA
Sample Human Serum (Serum)
Specification Serum
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Type Sandwich
Username

Abcam user community

Verified customer

投稿 Aug 08 2006

Thank you for your enquiry. Ab11081 reacts specifically with human plasma and cytoplasmic gelsolin in immunoblotting. The antibody is directed against an epitope located on the 47 kD peptide derived from a chymotryptic cleavage of gelsolin. This p...

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I can confirm that ab11081 will detect both cytoplasmic gelsolin and actin-bound gelsolin. Please do not hesitate to contact us again if you need further information,

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"