製品の概要

  • 製品名Anti-Gelsolin antibody [EPR1942]
    Gelsolin 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1942] to Gelsolin
  • アプリケーション適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details
    適用なし: IP
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) (internal sequence)

  • ポジティブ・コントロール
    • MCF-7, HL-60, A431, and HeLa cell lysates; Human kidney tissue; Human lung tissue; HeLa cells
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109014 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/50000 - 1/20000. Predicted molecular weight: 86 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/50 - 1/1000. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
ICC/IF 1/100 - 1/250.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 機能Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
    • 組織特異性Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
    • 関連疾患Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
    • 配列類似性Belongs to the villin/gelsolin family.
      Contains 6 gelsolin-like repeats.
    • 翻訳後修飾Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
    • 細胞内局在Cytoplasm > cytoskeleton and Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Actin depolymerizing factor antibody
      • Actin-depolymerizing factor antibody
      • ADF antibody
      • AGEL antibody
      • Brevin antibody
      • DKFZp313L0718 antibody
      • GELS_HUMAN antibody
      • Gelsolin antibody
      • Gsn antibody
      see all

    Anti-Gelsolin antibody [EPR1942] 画像

    • All lanes : Anti-Gelsolin antibody [EPR1942] (ab109014) at 1/20000 dilution

      Lane 1 : THP-1 whole cell lysate
      Lane 2 : THP-1 cultured medium
      Lane 3 : MCF-7 whole cell lysate
      Lane 4 : Human Plasma

      Lysates/proteins at 10 µg per lane.

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size : 86 kDa
      Observed band size : 81/86 kDa (why is the actual band size different from the predicted?)


      Exposure time : 10 seconds

      Blocking buffer 5% NFDM/TBST
      Diluting buffer 5% NFDM/TBST

      The secretory isoform of gelsolin is at 86kDa. The cytoplasmic isoforms of gelsolin are at 81kDa.

    • All lanes : Anti-Gelsolin antibody [EPR1942] (ab109014) at 1/50000 dilution

      Lane 1 : MCF7 cell lysate
      Lane 2 : HL60 cell lysate
      Lane 3 : A431 cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 86 kDa
    • Immunohistochemical analysis of Gelsolin expression in paraffin-embedded Human kidney tissue using ab109014 at 1/250 dilution.
    • Immunohistochemical analysis of Gelsolin expression in paraffin-embedded Human lung tissue using ab109014 at 1/250 dilution.
    • Immunofluorescent staining of Gelsolin in HeLa cells using ab109014 at 1/100 dilution.
    • Overlay histogram showing HL60 cells stained with ab109014 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109014, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HL60 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

    Anti-Gelsolin antibody [EPR1942] (ab109014) 使用論文

    This product has been referenced in:
    • Mazur AJ  et al. Analysis of gelsolin expression pattern in developing chicken embryo reveals high GSN expression level in tissues of neural crest origin. Brain Struct Funct N/A:N/A (2014). WB ; Chicken . Read more (PubMed: 25352156) »

    See 1 Publication for this product

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