製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Gelsolin
  • アプリケーション
    適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Sheep, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide within Human Gelsolin aa 542-591. The exact sequence is proprietary.
    Sequence:

    KPMIIYKGGTSREGGQTAPASTRLFQVRANSAGATRAVEVLPKAGALNSN


    Database link: P06396

  • ポジティブ・コントロール
    • MCF cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab76418 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 86 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
  • 組織特異性
    Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
  • 関連疾患
    Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
  • 配列類似性
    Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
  • 翻訳後修飾
    Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
  • 細胞内局在
    Cytoplasm > cytoskeleton and Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Actin depolymerizing factor antibody
    • Actin-depolymerizing factor antibody
    • ADF antibody
    • AGEL antibody
    • Brevin antibody
    • DKFZp313L0718 antibody
    • GELS_HUMAN antibody
    • Gelsolin antibody
    • Gsn antibody
    see all

画像

  • ab76418 at 5µg/ml staining Gelsolin in tonsil tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Anti-Gelsolin antibody (ab76418) at 1 µg/ml + MCF7 cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 86 kDa
    Observed band size : 86 kDa


  • Predicted band size : 86 kDa
    Commercial M1 and M2 are cell culture supernatants of human monocyte derived macrophages.

参考文献

This product has been referenced in:
  • Bewley MA  et al. Proteomic evaluation and validation of cathepsin D regulated proteins in macrophages exposed to Streptococcus pneumoniae. Mol Cell Proteomics 10:M111.008193 (2011). WB ; Human . Read more (PubMed: 21474794) »
  • Haverland N  et al. Immunoreactivity of anti-gelsolin antibodies: implications for biomarker validation. J Transl Med 8:137 (2010). WB ; Human . Read more (PubMed: 21171974) »

See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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