製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Gelsolin
  • アプリケーション
    適用あり: WB, IHC-P, ICC, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Cow
  • 免疫原

    Recombinant fragment within Human Gelsolin aa 161-369. The exact sequence is proprietary.
    Database link: P06396

  • ポジティブ・コントロール
    • Human Tonsil tissue; H1299 and Hela whole cell lysates; HeLa cells.

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 79% Tris glycine, 20% Glycerol
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab113229 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/3000. Predicted molecular weight: 86 kDa.
IHC-P Use a concentration of 10 µg/ml.
ICC 1/100 - 1/200.
ICC/IF 1/100 - 1/200.

ターゲット情報

  • 機能
    Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
  • 組織特異性
    Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
  • 関連疾患
    Defects in GSN are the cause of amyloidosis type 5 (AMYL5) [MIM:105120]; also known as familial amyloidosis Finnish type. AMYL5 is a hereditary generalized amyloidosis due to gelsolin amyloid deposition. It is typically characterized by cranial neuropathy and lattice corneal dystrophy. Most patients have modest involvement of internal organs, but severe systemic disease can develop in some individuals causing peripheral polyneuropathy, amyloid cardiomyopathy, and nephrotic syndrome leading to renal failure.
  • 配列類似性
    Belongs to the villin/gelsolin family.
    Contains 6 gelsolin-like repeats.
  • 翻訳後修飾
    Phosphorylation on Tyr-86, Tyr-409, Tyr-465, Tyr-603 and Tyr-651 in vitro is induced in presence of phospholipids.
  • 細胞内局在
    Cytoplasm > cytoskeleton and Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Actin depolymerizing factor antibody
    • Actin-depolymerizing factor antibody
    • ADF antibody
    • AGEL antibody
    • Brevin antibody
    • DKFZp313L0718 antibody
    • GELS_HUMAN antibody
    • Gelsolin antibody
    • Gsn antibody
    see all

画像

  • ab113229, at 10µg/ml, staining Gelsolin in Formalin-fixed, Paraffin-embedded Human Tonsil tissue by Immunohistochemistry.
  • All lanes : Anti-Gelsolin antibody (ab113229) at 1/1000 dilution

    Lane 1 : H1299 whole cell lysate
    Lane 2 : HeLa whole cell lysate

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 86 kDa
  • ab113229 at 1/200 dilution staining Gelsolin in methanol-fixed HeLa cells by Immunofluorescence. The lower image is co-stained with Hoeschst 33342.

参考文献

ab113229 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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