製品の概要

  • 製品名Anti-GEFT antibody
    GEFT 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GEFT
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Dog, Pig, Chimpanzee, Spider Monkey
  • 免疫原

    Synthetic peptide corresponding to a region within internal amino acids 504-553 (RIRLGDQAQGSTHTPINGSLPSLLLSPKGEVARALLPLDKQALGDIPQA P) of Human GEFT (NP_891992).

  • ポジティブ・コントロール
    • Fetal brain lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 64 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1:312500.

ターゲット情報

  • 機能May play a role in actin cytoskeleton reorganization in different tissues since its activation induces formation of actin stress fibers. It works as a guanine nucleotide exchange factor for Rho family of small GTPases. Links specifically G alpha q/11-coupled receptors to RHOA activation. May be an important regulator of processes involved in axon and dendrite formation. In neurons seems to be an exchange factor primarily for RAC1. Involved in skeletal myogenesis.
  • 組織特異性Isoform 1 and isoform 2 are highly expressed in excitable tissues, such as brain, heart and muscle. Also detected in kidney and liver.
  • 配列類似性Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
  • ドメインThe guanine nucleotide exchange activity is autoinhibited by the PH domain.
  • 細胞内局在Cell membrane. Cytoplasm > myofibril > sarcomere. Highly colocalizes with actin regions.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARHGEF25 antibody
    • ARHGP_HUMAN antibody
    • GEFT antibody
    • Guanine nucleotide exchange factor GEFT antibody
    • p63RhoGEF antibody
    • RAC/CDC42 exchange factor antibody
    • Rac/Cdc42/Rho exchange factor GEFT antibody
    • Rho guanine nucleotide exchange factor 25 antibody
    • RhoA/RAC/CDC42 exchange factor antibody
    • RhoA/Rac/Cdc42 guanine nucleotide exchange factor GEFT antibody
    see all

Anti-GEFT antibody 画像

  • Anti-GEFT antibody (ab81684) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal brain lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG should be diluted 1/50,000 - 1/100,000 as secondary antibody.

    Predicted band size : 64 kDa
    Observed band size : 64 kDa
    Additional bands at : 65 kDa,70 kDa. We are unsure as to the identity of these extra bands.

Anti-GEFT antibody (ab81684) 使用論文

ab81684 has not yet been referenced specifically in any publications.

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