Anti-GEF H1 (phospho S885) 抗体 (ab74156)

製品の概要

  • 製品名Anti-GEF H1 (phospho S885) antibody
    GEF H1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GEF H1 (phospho S885)
  • 特異性ab74156 detects endogenous levels of GEF H1 only when phosphorylated at serine 885.
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic phosphopeptide derived from human GEF H1 around the phosphorylation site of serine 885 (R-R-SP-L-P).

  • ポジティブ・コントロール
    • Extracts from HeLa cells treated with TSA (400nM, 24hours) and Jurkat cells treated with forskolin (40nM, 30mins).

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab74156 was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab74156 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Predicted molecular weight: 120 kDa.
ELISA 1/5000.

ターゲット情報

  • 機能Activates Rho-GTPases by promoting the exchange of GDP for GTP. May be involved in epithelial barrier permeability, cell motility and polarization, dendritic spine morphology, antigen presentation, leukemic cell differentiation, cell cycle regulation, and cancer. Binds Rac-GTPases, but does not seem to promote nucleotide exchange activity toward Rac-GTPases, which was uniquely reported in PubMed:9857026. May stimulate instead the cortical activity of Rac. Inactive toward CDC42, TC10, or Ras-GTPases. Forms an intracellular sensing system along with NOD1 for the detection of microbial effectors during cell invasion by pathogens. Required for RHOA and RIP2 dependent NF-kappaB signaling pathways activation upon S.flexneri cell invasion. Involved not only in sensing peptidoglycan (PGN)-derived muropeptides through NOD1 that is independent of its GEF activity, but also in the activation of NF-kappaB by Shigella effector proteins (IpgB2 and OspB) which requires its GEF activity and the activation of RhoA.
  • 配列類似性Contains 1 DH (DBL-homology) domain.
    Contains 1 PH domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
  • ドメインThe DH (DBL-homology) domain interacts with and promotes loading of GTP on RhoA.
    The PH (pleckstrin-homology) domain is involved in microtubule binding and targeting to tight junctions.
  • 翻訳後修飾Phosphorylation of Ser-886 by PAK1 induces binding to protein 14-3-3 zeta, promoting its relocation to microtubules and the inhibition of its activity. Phosphorylated by STK6 and CDK1 during mitosis, which negatively regulates its activity. Phosphorylation by MAPK1 or MAPK3 increases nucleotide exchange activity. Phosphorylation by PAK4 releases GEF-H1 from the microtubules.
  • 細胞内局在Cytoplasm. Cell junction > tight junction. Golgi apparatus. Cytoplasm > cytoskeleton > spindle. Cell projection > ruffle membrane. Localizes to the tips of cortical microtubules of the mitotic spindle during cell division, and is further released upon microtubule depolymerization. Recruited into membrane ruffles induced by S.flexneri at tight junctions of polarized epithelial cells.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AA408978 antibody
    • ARHG2 antibody
    • ARHG2_HUMAN antibody
    • ARHGEF 2 antibody
    • ARHGEF-2 antibody
    • ARHGEF2 antibody
    • GEF antibody
    • GEF H1 antibody
    • GEF-H1 antibody
    • GEFH1 antibody
    • Guanine nucleotide exchange factor H1 antibody
    • KIAA0651 antibody
    • Lbcl1 antibody
    • Lfc antibody
    • LFP40 antibody
    • MGC95068 antibody
    • Microtubule-regulated Rho-GEF antibody
    • mKIAA0651 antibody
    • P40 antibody
    • Proliferating cell nucleolar antigen p40 antibody
    • Protein GEF-H1 antibody
    • Rho guanine nucleotide exchange factor 2 antibody
    • rho/rac guanine nucleotide exchange factor (GEF) 2 antibody
    • rho/rac guanine nucleotide exchange factor 2 antibody
    • rho/rac guanine nucleotide exchange factor antibody
    see all

Anti-GEF H1 (phospho S885) antibody 画像

  • All lanes : Anti-GEF H1 (phospho S885) antibody (ab74156) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells treated with TSA (400nM, 24hours)
    Lane 2 : Extracts from Jurkat cells treated with forskolin (40nM, 30mins)
    Lane 3 : Extracts from HeLa cells treated with TSA (400nM, 24hours) with immunising phosphopeptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 120 kDa
    Observed band size : 115 kDa (why is the actual band size different from the predicted?)

Anti-GEF H1 (phospho S885) antibody (ab74156) 使用論文

ab74156 has not yet been referenced specifically in any publications.

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Thank you for your inquiry and your patience with this reply.

The immunogens for ab74156, ab79175 and ab94348 all share 100% homology with xenopus.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"