製品の概要

  • 製品名Anti-GDNF Receptor alpha 1 antibody
    GDNF Receptor alpha 1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GDNF Receptor alpha 1
  • アプリケーション適用あり: IHC (PFA fixed), IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide, corresponding to amino acids 369-382 of Human GFR alpha 1.

  • ポジティブ・コントロール
    • Human brain lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度IgG fraction
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab8026 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC (PFA fixed)
IHC-P
WB
  • 追加情報IHC-P: Use at a concentration of 1 µg/ml. Perform heat mediated antigen retrieval (citrate buffer 10 mM, pH 6.0) before commencing with IHC staining protocol.
    Immunohistochemistry (PFA fixed): 1/50.
    WB: 1/500. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Receptor for GDNF. Mediates the GDNF-induced autophosphorylation and activation of the RET receptor.
    • 配列類似性Belongs to the GDNFR family.
    • 細胞内局在Cell membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • GDNF family receptor alpha 1 antibody
      • GDNF family receptor alpha-1 antibody
      • GDNF R antibody
      • GDNF RA antibody
      • GDNF receptor alpha antibody
      • GDNF receptor alpha-1 antibody
      • GDNFR alpha 1 antibody
      • GDNFR alpha antibody
      • GDNFR antibody
      • GDNFR-alpha-1 antibody
      • GDNFRA antibody
      • GDNFRalpha antibody
      • GFR alpha 1 antibody
      • GFR alpha1 antibody
      • GFR-alpha-1 antibody
      • GFRA 1 antibody
      • Gfra1 antibody
      • GFRA1_HUMAN antibody
      • GFRalpha1 antibody
      • Glial cell line derived neurotrophic factor receptor alpha antibody
      • GPI linked anchor protein antibody
      • MGC23045 antibody
      • PI linked cell surface accessory protein antibody
      • RET 1L antibody
      • RET ligand 1 antibody
      • RET1L antibody
      • RETL 1 antibody
      • RETL1 antibody
      • TGF beta related neurotrophic factor receptor 1 antibody
      • TGF-beta-related neurotrophic factor receptor 1 antibody
      • TRNR 1 antibody
      • TRNR1 antibody
      see all

    Anti-GDNF Receptor alpha 1 antibody 画像

    • All lanes : Anti-GDNF Receptor alpha 1 antibody (ab8026) at 1/500 dilution

      Lane 1 : Crude membrane fractions of human brain
      Lane 2 : Crude membrane fractions of liver
      Lane 3 : Crude membrane fractions of kidney
      Lane 4 : Crude membrane fractions of spleen


      Predicted band size : 50 kDa
      Observed band size : 50 kDa

    Anti-GDNF Receptor alpha 1 antibody (ab8026) 使用論文

    This product has been referenced in:
    • Bellaïche J  et al. Gdnf-Gfra1 pathway is expressed in a spermatogenetic-dependent manner and is regulated by fsh in a fish testis. Biol Reprod 91:94 (2014). Read more (PubMed: 25165121) »
    • He Z  et al. Isolation, characterization, and culture of human spermatogonia. Biol Reprod 82:363-72 (2010). IHC-P ; Human . Read more (PubMed: 19846602) »

    See all 3 Publications for this product

    Product Wall

    Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample Rat Tissue sections (Brain)
    Specification Brain
    Fixative Paraformaldehyde
    Antigen retrieval step None
    Permeabilization Yes - 0.1% triton x-100
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 20°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 13 2008

    Thank you for your enquiry. I can confirm that the epitope of ab8026 GDNF Receptor alpha 1 antibody is predicted to be extracellular. We recommend a citrate antigen retrieval method for this antibody. I hope this is helpful. Should you have any ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"