製品の概要

  • 製品名Anti-GDNF antibody
    GDNF 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GDNF
  • 特異性GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot.
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Chicken, Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 150 to the C-terminus of Mouse GDNF.

    (Peptide available as ab74004.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following lysates: HepG2 Whole Cell Mouse Liver Tissue Human Pancreas Tissue

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab64337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 24 kDa).

GDNF homodimerizes and has 5 isoforms along with a signal and propeptide, so there is a potential for multiple bands in western blot.

ターゲット情報

  • 機能Neurotrophic factor that enhances survival and morphological differentiation of dopaminergic neurons and increases their high-affinity dopamine uptake.
  • 組織特異性In the brain, predominantly expressed in the striatum with highest levels in the caudate and lowest in the putamen.
  • 関連疾患Defects in GDNF may be a cause of Hirschsprung disease (HSCR) [MIM:142623]. In association with mutations of RET gene, defects in GDNF may be involved in Hirschsprung disease. This genetic disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction.
    Defects in GDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia.
  • 配列類似性Belongs to the TGF-beta family. GDNF subfamily.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Astrocyte derived trophic factor antibody
    • Astrocyte derived trophic factor 1 antibody
    • Astrocyte-derived trophic factor antibody
    • ATF 1 antibody
    • ATF 2 antibody
    • Atf antibody
    • ATF1 antibody
    • ATF2 antibody
    • gdnf antibody
    • GDNF_HUMAN antibody
    • Glial cell derived neurotrophic factor antibody
    • Glial Cell Line Derived Neurotrophic Factor antibody
    • Glial cell line-derived neurotrophic factor antibody
    • Glial derived neurotrophic factor antibody
    • HFB1 GDNF antibody
    • hGDNF antibody
    • HSCR3 antibody
    see all

Anti-GDNF antibody 画像

  • All lanes : Anti-GDNF antibody (ab64337) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Liver (Mouse) Tissue Lysate
    Lane 3 : Human pancreas tissue lysate - total protein (ab29816)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 24 kDa
    Observed band size : 24 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes

Anti-GDNF antibody (ab64337) 使用論文

ab64337 has not yet been referenced specifically in any publications.

Product Wall

Thank you very much for your reply and for the additional information.

Please keep me updated about any new results after heating the samples for 10 min and diluting the antibody to 1:1000.

I look forward to hearing from you, and le...

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Thank you for your reply and for sending the additional information. I apologize for the delay getting back to you, as our office was closed yesterday for Memorial Day.

Could you also tell me how longthe samples wereboiled in sample buffer, a...

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Thank you for your call today and for sending the image and information.

The band around 25 kDa is probably the expected monomer of GDNF, and the 50 kDa band is most likely the dimerized form of the protein. How long were the samples boiled i...

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Mouse Cell (embryonic kidney)
Specification embryonic kidney
Fixative PFA or MeOH or Acetone
Permeabilization No
Blocking step Serum as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
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Dr. Odysse Michos

Verified customer

投稿 May 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"