製品の概要

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab98337 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).

ターゲット情報

Anti-GDF8 / Myostatin antibody 画像

  • All lanes : Anti-GDF8 / Myostatin antibody (ab98337) at 1/250 dilution

    Lane 1 : Human skeletal muscle tissue lysate - total protein (ab29330)
    Lane 2 : Skeletal Muscle (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa
    Observed band size : 43 kDa
    Additional bands at : 28 kDa (possible cleavage fragment).

    Exposure time : 16 minutes
  • All lanes : Anti-GDF8 / Myostatin antibody (ab98337) at 1/250 dilution

    Lane 1 : Recombinant human GDF8 / Myostatin protein (ab73251) at 0.1 µg
    Lane 2 : Recombinant human GDF8 / Myostatin protein (ab73251) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 43 kDa


    Exposure time : 30 seconds

    ab73251 is a homodimer consisting of two 109aa monomers. The homodimer expected size is 25kDa, so the observed band is thought to be the monomer form.

Anti-GDF8 / Myostatin antibody (ab98337) 使用論文

This product has been referenced in:
  • Morrison PK  et al. Post-mortem stability of RNA in skeletal muscle and adipose tissue and the tissue-specific expression of myostatin, perilipin and associated factors in the horse. PLoS One 9:e100810 (2014). WB ; Horse . Read more (PubMed: 24956155) »
  • Morrison PK  et al. Preliminary investigation into a potential role for myostatin and its receptor (ActRIIB) in lean and obese horses and ponies. PLoS One 9:e112621 (2014). WB ; Horse . Read more (PubMed: 25390640) »

See all 3 Publications for this product

Product Wall

Application Western blot
Loading amount 40 µg
Gel Running Conditions Reduced Non-Denaturing (Native)
Sample Human Cell lysate - whole cell (Gastrocnemius)
Specification Gastrocnemius
Blocking step (agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 26°C
Username

Dr. FRANCESCO ELIA MARINO

Verified customer

投稿 Mar 12 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing (10 % gel)
Sample Chicken Tissue lysate - whole (skeletal muscle)
Specification skeletal muscle
Blocking step BSA as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Abcam user community

Verified customer

投稿 Jul 17 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Horse Tissue lysate - whole (skeletal muscle)
Loading amount 45 µg
Specification skeletal muscle
Gel Running Conditions Reduced Denaturing (4%stacking, 10% running)
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Pippa Morrison

Verified customer

投稿 Nov 12 2012

That is no problem. If you have any further questions, please do not hesitate to contact us.


As you are interested in participating in the testing discount scheme, I have generated a discount code for you to use (See below). Once you ha...

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Thank you for contacting us.


I have performed a sequence comparison of the peptide immunogen used to raise this antibody to that of equine myostatin (SwissProt reference http://www.uniprot.org/uniprot/Q9GM97). The sequences match 100%, ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"