製品の概要

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPreservative: 0.09% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • 精製度Ammonium Sulphate Precipitation
  • 特記事項(精製)This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab71808 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P 1/50.

ターゲット情報

Anti-GDF8 / Myostatin antibody 画像

  • Anti-GDF8 / Myostatin antibody (ab71808) at 1/2000 dilution (Anti-GDF8 Antibody (N24)) + Human skeletal muscle lysate at 20 µg

    Secondary
    Goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/10000 dilution

    Predicted band size : 43 kDa
    Observed band size : 43 kDa

    Blocking/Dilution buffer: 5% NFDM/TBST

  • Immunohistochemical staining of GDF8 / Myostatin in Human skeletal muscle tissue sections (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections) with ab71808 at a dilution of 1/25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0.5 hours at 38°C. antigen retrieval was heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37°C. A HRP-conjugated goat anti-rabbit polyclonal (ready to use) was used as the secondary antibody.

Anti-GDF8 / Myostatin antibody (ab71808) 使用論文

This product has been referenced in:
  • Marino FE  et al. Activin-ßC modulates cachexia by repressing the ubiquitin-proteasome and autophagic degradation pathways. J Cachexia Sarcopenia Muscle 6:365-80 (2015). WB ; Mouse . Read more (PubMed: 26673867) »

See 1 Publication for this product

Product Wall

Application Western blot
Loading amount 50 µg
Gel Running Conditions Non-reduced Non-Denaturing (Native) (gel 12%)
Sample Human Recombinant protein (Source:Mouse myeloma cell line, NS0derived)
Specification Source:Mouse myeloma cell line, NS0derived
Treatment 65.8 mM Tris-HCl, pH 6.8, 2.1% SDS, 26.3% (w/v) glycerol, 0.01% bromophenol blue + 54mM DTT (lemmli buffer + DTT)
Blocking step Odyssey Blocking Buffer (927-40000) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 0% · Temperature: 22°C
Username

Dr. FRANCESCO ELIA MARINO

Verified customer

投稿 Dec 24 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"