Rabbit polyclonal to GDF3
Synthetic peptide from the center region of human GDF3 conjugated to KLH
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Preservative: 0.09% Sodium Azide
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Ammonium Sulphate Precipitation
ab71250 is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
1/10 - 1/50.
Belongs to the TGF-beta family.
Information by UniProt
GDF 3 antibody
Western blot - GDF3 antibody (ab71250)
Anti-GDF3 antibody (ab71250) at 1/60 dilution + HepG2 cell line lysates at 35 µg
Predicted band size : 41 kDa Observed band size : 41 kDa Additional bands at : 25 kDa,60 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-GDF3 antibody(ab71250)
ab71250 at 1/50 dilution staining GDF3 in human testis tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded sections). A peroxidase conjugated secondary antibody was used followed by DAB staining.
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