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Recombinant MBP-GCSFR fusion protein (Human).
Abcam is committed to meeting high standards of ethical manufacturing and as such, we will be discontinuing this product, which has been generated by the ascites method, within the next year. We are sorry for any inconvenience this may cause. If you would like help finding an alternative product, please do not hesitate to contact our scientific support team.
Our Abpromise guarantee covers the use of ab18627 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent dilution.|
|WB||Use at an assay dependent dilution. Predicted molecular weight: 92 kDa.|
|ICC/IF||1/500. See Abreview.|
ab18627 staining human Bone Marrow cells by ICC/IF. Cells were PFA fixed and permeabilized in 1% Triton X-100 prior to blocking in 0.5% BSA for 1 hour at 25°C. The primary antibody was diluted 1/500 and incubated with the sample for 2 hours at 25°C. A FITC conjugated goat anti-mouse antibody, diluted 1/100, was used as the secondary. The image on the left shows the 40X view of Bone Marrow cells stained for GCSFR showing the FITC stain predominantly on cell membranes. The righthand image is the same image with DAPI overlay to show the cell nuclei.
ab18627 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"