製品の概要

  • 製品名Anti-GCS1 antibody
    GCS1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GCS1
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Dog, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 144-193 (GPYGWEFHDG LSFGRQHIQD GALRLTTEFV KRPGGQHGGD WSWRVTVEPQ) of human GCS1 (NP_006293).

  • ポジティブ・コントロール
    • HeLa cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)ab82962 is purified by a peptide affinity chromatography method.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab82962 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 92 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay, 1:312500.

ターゲット情報

  • 機能Cleaves the distal alpha 1,2-linked glucose residue from the Glc(3)Man(9)GlcNAc(2) oligosaccharide precursor in a highly specific manner.
  • パスウェイGlycan metabolism; N-glycan degradation.
  • 関連疾患Defects in MOGS are the cause of type IIb congenital disorder of glycosylation (CDGIIb) [MIM:606056]; also known as glucosidase I deficiency. CDGIIb is characterized by marked generalized hypotonia and hypomotility of the neonate, dysmorphic features, including a prominent occiput, short palpebral fissures, retrognathia, high arched palate, generalized edema, and hypoplastic genitalia. Symptoms of the infant included hepatomegaly, hypoventilation, feeding problems and seizures. The clinical course was progressive and the infant did not survive more than a few months.
  • 配列類似性Belongs to the glycosyl hydrolase 63 family.
  • 細胞内局在Endoplasmic reticulum membrane.
  • Information by UniProt
  • 参照データベース
  • 別名
    • EC 3.2.1.106 antibody
    • glucosidase I antibody
    • Mannosyl oligosaccharide glucosidase antibody
    • Mannosyl-oligosaccharide glucosidase antibody
    • Mogs antibody
    • MOGS_HUMAN antibody
    • Processing A glucosidase I antibody
    • Processing A-glucosidase I antibody
    see all

Anti-GCS1 antibody 画像

  • Anti-GCS1 antibody (ab82962) at 1 µg/ml + HeLa cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 92 kDa
    Observed band size : 92 kDa
    Additional bands at : 24 kDa,50 kDa. We are unsure as to the identity of these extra bands.

Anti-GCS1 antibody (ab82962) 使用論文

ab82962 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"