製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GCP6
  • アプリケーション
    適用あり: WB, IP, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Orangutan
  • 免疫原

    Synthetic peptide, corresponding to a region within amino acids 1350-1400 of Human GCP6, NP_065194.2

  • ポジティブ・コントロール
    • WB: HeLa and 293T cell lysates. IP: HeLa cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG

アプリケーション

Our Abpromise guarantee covers the use of ab95172 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 200 kDa.
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 関連性
    The protein encoded by this gene is part of a large multisubunit complex required for microtubule nucleation at the centrosome.
  • 細胞内局在
    Cytoplasmic
  • 参照データベース
  • 別名
    • Gamma tubulin complex component 6 antibody
    • GCP 6 antibody
    • MCCRP antibody
    • MCPHCR antibody
    • TUBGCP6 antibody
    • tubulin, gamma complex associated protein 6 antibody
    see all

画像

  • ICC/IF image of ab95172 stained HepG2 cells.  The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab95172 at 1 µg/mL overnight at +4°C. The secondary antibody (green) was DyLight® 488 Goat anti-Rabbit IgG (H+L) (ab96899) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-GCP6 antibody (ab95172) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T whole cell lysate at 50 µg


    Predicted band size : 200 kDa
  • Lane 1: 3µg ab95172 were used to immunoprecipitate GCP3 from 1mg HeLa whole cell lysate. 20% IP was loaded per lane and probed with ab95170 at 1µg/ml.
    Lane 2: same, using a control IgG.
    Detection: chemiluminescence with exposure time of 30 seconds.

参考文献

This product has been referenced in:
  • Farache D  et al. Functional Analysis of ?-Tubulin Complex Proteins Indicates Specific Lateral Association via Their N-terminal Domains. J Biol Chem 291:23112-23125 (2016). Read more (PubMed: 27660388) »
  • Lecland N & Lüders J The dynamics of microtubule minus ends in the human mitotic spindle. Nat Cell Biol 16:770-8 (2014). Read more (PubMed: 24976384) »

See all 3 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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