The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 52 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
機能Plays a role in cellular signaling via Rho-related GTP-binding proteins and subsequent activation of transcription factor SRF (By similarity). Targets TJP1 to cell junctions. In cortical neurons, may play a role in glutaminergic signal transduction through interaction with the NMDA receptor subunit GRIN1.
組織特異性Detected in heart, liver, skeletal muscle, placenta, small intestine, lung, prostate and testis.
配列類似性Belongs to the MYZAP family.
細胞内局在Cytoplasm > cytoskeleton. Cell membrane. Cytoplasm > myofibril > sarcomere > I band. Cytoplasm > myofibril > sarcomere > Z line. Cell junction. Detected predominantly at the intercalated disk in cardiomyocytes, and at low levels on sarcomeric Z disks. Colocalizes with F-actin. Colocalizes with cortical actin.