The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).
Use at an assay dependent dilution.
GCN5p is a histone acetyltransferase that contains a bromo domain and an N-acetyltransferase domain. It is recruited by a number of DNA-binding transcriptional activators, such as GCN4p and HAP2/3/4. GCN5p is a component of a number of different histone acetylation (HAT) complexes, namely, SAGA, SALSA and ADA. The SAGA complex, which is around 1.8 MDa in size, is required for the recruitment of the basal transcriptional machinery to around 10% of RNA polymerase II gene promoters in yeast. The activity of GCN5 may be dependent on its interactions within these multisubunit complexes. GCN5p acetylates histone H2B on K11 and K16 and histone H3 on K9, K14, K18, K23, K27 and K36. It also contributes to acetylation of histone H4 (at K8 and K16) and histone H2A.Z.
Bian C et al. Sgf29 binds histone H3K4me2/3 and is required for SAGA complex recruitment and histone H3 acetylation. EMBO J30:2829-42 (2011).
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