製品の概要

  • 製品名Anti-GCN5p antibody
  • 製品の詳細
    Rabbit polyclonal to GCN5p
  • アプリケーション適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Saccharomyces cerevisiae
  • 免疫原

    Recombinant fragment corresponding to the N-terminal domain (1-300aa) of Saccharomyces cerevisiae GCN5p.

  • ポジティブ・コントロール
    • Saccharomyces cerevisiae extract.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab63810 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/5000. Detects a band of approximately 54 kDa (predicted molecular weight: 51 kDa).
ELISA Use at an assay dependent dilution.

ターゲット情報

  • 関連性GCN5p is a histone acetyltransferase that contains a bromo domain and an N-acetyltransferase domain. It is recruited by a number of DNA-binding transcriptional activators, such as GCN4p and HAP2/3/4. GCN5p is a component of a number of different histone acetylation (HAT) complexes, namely, SAGA, SALSA and ADA. The SAGA complex, which is around 1.8 MDa in size, is required for the recruitment of the basal transcriptional machinery to around 10% of RNA polymerase II gene promoters in yeast. The activity of GCN5 may be dependent on its interactions within these multisubunit complexes. GCN5p acetylates histone H2B on K11 and K16 and histone H3 on K9, K14, K18, K23, K27 and K36. It also contributes to acetylation of histone H4 (at K8 and K16) and histone H2A.Z.
  • 細胞内局在Nuclear
  • 参照データベース
    • 別名
      • ADA4 antibody
      • GCN 5p antibody
      • GCN5 antibody
      • Histone acetyltransferase GCN5 antibody
      • SWI9 antibody
      see all

    Anti-GCN5p antibody 画像

    • Anti-GCN5p antibody (ab63810) at 1/500 dilution + Lysate from S. cerevisiae(BY4741)

      Secondary
      HRP conjugated Goat anti-Rabbit IgG at 1/5000 dilution

      Predicted band size : 51 kDa
    • All lanes : Anti-GCN5p antibody (ab63810) at 1/5000 dilution

      Lane 1 : Molecular weight markers
      Lane 2 : Saccharomyces cerevisiae extract


      Predicted band size : 51 kDa
      Observed band size : 54 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 34 kDa,50 kDa. We are unsure as to the identity of these extra bands.

    Anti-GCN5p antibody (ab63810) 使用論文

    ab63810 has not yet been referenced specifically in any publications.

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