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RabMAb

Anti-GCLM 抗体 [EPR6668] (ab124827)

Abreviews (1)Q&A (1)Specific References (3)

製品の概要

  • 製品名
    Anti-GCLM antibody [EPR6668]
    GCLM 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR6668] to GCLM
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human GCLM aa 200-300. The exact sequence is proprietary.

  • ポジティブ･コントロール
    • HeLa, A673 and K562 cell lysates; Human muscle and liver tissue. Rat kidney. NIH/3T3 (mouse embryo) whole cell lysate. K562 (human chronic myelogenous leukemia) whole cell lysate.
  • 特記事項

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab124827 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 31 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cyt 1/100 - 1/500.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 
ICC/IF 1/250 - 1/500.

ターゲット情報

  • 組織特異性
    In all tissues examined. Highest levels in skeletal muscle.
  • パスウェイ
    Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
  • 配列類似性
    Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
    • Gamma ECS regulatory subunit antibody
    • Gamma-ECS regulatory subunit antibody
    • Gamma-glutamylcysteine synthetase regulatory subunit antibody
    • GCLM antibody
    • GCS light chain antibody
    • GLCLR antibody
    • Glutamate cysteine ligase regulatory subunit antibody
    • Glutamate--cysteine ligase modifier subunit antibody
    • Glutamate--cysteine ligase regulatory subunit antibody
    • GSC light chain antibody
    • GSH0_HUMAN antibody
    see all

画像

  • Immunoprecipitation - Anti-GCLM antibody [EPR6668] (ab124827)
    Immunoprecipitation - Anti-GCLM antibody [EPR6668] (ab124827)

    ab124827 immunoprecipitating GCLM. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/50 and VeriBlot for IP secondary antibody (HRP) (ab131366) at a dilution of 1/10000.

    Lane 1: K562 (human chronic myelogenous leukemia) whole cell lysate (10ug)
    Lane 2: K562 (human chronic myelogenous leukemia) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab124827 in K562 (human chronic myelogenous leukemia) whole cell lysate

  • Flow Cytometry - Anti-GCLM antibody [EPR6668] (ab124827)
    Flow Cytometry - Anti-GCLM antibody [EPR6668] (ab124827)

    ab124827 staining GCLM in HeLa (human cervix adenocarcinoma) cells by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.

    Isoytype control: Rabbit monoclonal IgG (Black)

    Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)

  • Immunocytochemistry/ Immunofluorescence - Anti-GCLM antibody [EPR6668] (ab124827)
    Immunocytochemistry/ Immunofluorescence - Anti-GCLM antibody [EPR6668] (ab124827)

    ab124827 staining GCLM in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 (1/1000) and ab150120 (1/1000) were used as counterstains for primary antibody ab124827 and secondary antibody ab150077 respectively. DAPI was used as a nuclear counterstain.

    Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
    Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077)

  • Western blot - Anti-GCLM antibody [EPR6668] (ab124827)
    Western blot - Anti-GCLM antibody [EPR6668] (ab124827)
    Anti-GCLM antibody [EPR6668] (ab124827) at 1/10000 dilution + NIH/3T3 (mouse embryo) whole cell lysate at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG)

    Predicted band size: 31 kDa



    Diluting and blocking buffer: 5% NFDM /TBST 

  • Immunohistochemistry - Anti-GCLM antibody [EPR6668] (ab124827)
    Immunohistochemistry - Anti-GCLM antibody [EPR6668] (ab124827)

    ab124827 staining GCLM in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.

    Negative control 1: PBS in place of primary antibody.

  • Western blot - Anti-GCLM antibody [EPR6668] (ab124827)
    Western blot - Anti-GCLM antibody [EPR6668] (ab124827)
    All lanes : Anti-GCLM antibody [EPR6668] (ab124827) at 1/10000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
    Lane 2 : Human skeletal muscle
    Lane 3 : Rat kidney tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

    Predicted band size: 31 kDa



    Diluting and blocking buffer: 5% NFDM/TBST

  • Western blot - Anti-GCLM antibody [EPR6668] (ab124827)
    Western blot - Anti-GCLM antibody [EPR6668] (ab124827)
    All lanes : Anti-GCLM antibody [EPR6668] (ab124827) at 1/1000 dilution

    Lane 1 : HeLa cell lysate
    Lane 2 : A673 cell lysate
    Lane 3 : K562 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : HRP conjugated goat anti-rabbit at 1/2000 dilution

    Predicted band size: 31 kDa
    Observed band size: 30 kDa (why is the actual band size different from the predicted?)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6668] (ab124827)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6668] (ab124827)
    ab124827, at 1/100 dilution, staining GCLM in paraffin-embedded Human muscle tissue by Immunohistochemistry.
  • Other - Anti-GCLM antibody [EPR6668] (ab124827)
    Other - Anti-GCLM antibody [EPR6668] (ab124827)
    Equilibrium disassociation constant (KD)
    Learn more about KD

    Click here to learn more about KD

参考文献

This product has been referenced in:
  • Huang M  et al. Brd4 regulates the expression of essential autophagy genes and Keap1 in AML cells. Oncotarget 9:11665-11676 (2018). Read more (PubMed: 29545928) »
  • Dehghan E  et al. Hydralazine induces stress resistance and extends C. elegans lifespan by activating the NRF2/SKN-1 signalling pathway. Nat Commun 8:2223 (2017). Read more (PubMed: 29263362) »

See all 3 Publications for this product

ab124827 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

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2 reviews or Q&A

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Western blot abreview for Anti-GCLM antibody [EPR6668]

 Average
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (ARPE-19)
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Loading amount
10 µg
Treatment
50 uM 4-HNE, for 12-24 h
Specification
ARPE-19
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Abcam user community

Verified customer

投稿 Mar 22 2017

Name: jiao guangzhong
Product code: 124827

Inquiry: I want to kown if this product can be used to test gclm ptotein in mouse oocyte by immunofluorescence. thank you !

Thank you for contacting us.

ab124827 has been tested in mouse and immunocytochemistry (ICC) so it should be suitable for your experiment needs.

I hope this information is helpful to you. Please do not hesitate to contact us if yo...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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