製品の概要
-
製品名Anti-GCLM antibody [EPR6668]
GCLM 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EPR6668] to GCLM
-
由来種Rabbit
-
アプリケーション適用あり: WB, IP, IHC-P, Flow Cyt, ICC/IFmore details
-
種交差性交差種: Mouse, Rat, Human
-
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human GCLM aa 200-300.
-
ポジティブ･コントロール
- HeLa, A673 and K562 cell lysates; Human muscle and liver tissue. Rat kidney. NIH/3T3 (mouse embryo) whole cell lysate. K562 (human chronic myelogenous leukemia) whole cell lysate.
-
特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
This product is a recombinant rabbit monoclonal antibody.
製品の特性
-
製品の状態Liquid
-
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
-
解離定数（KD 値）KD = 3.89 x 10 -10 M Learn more about KD
-
バッファーpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol, 0.21% BSA, 59% PBS -
Concentration information loading... -
精製度Protein A purified
-
ポリ/モノモノクローナル
-
クローン名EPR6668
-
アイソタイプIgG
-
研究分野
関連製品
-
Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab124827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 30 kDa (predicted molecular weight: 31 kDa). | |
| IP | 1/10 - 1/100. | |
| IHC-P | 1/50 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| Flow Cyt | 1/100 - 1/500. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|
|
| ICC/IF | 1/250 - 1/500. |
ターゲット情報
-
組織特異性In all tissues examined. Highest levels in skeletal muscle.
-
パスウェイSulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.
-
配列類似性Belongs to the aldo/keto reductase family. Glutamate--cysteine ligase light chain subfamily.
- Information by UniProt
-
参照データベース
- Entrez Gene: 2730 Human
- Entrez Gene: 14630 Mouse
- Entrez Gene: 29739 Rat
- Omim: 601176 Human
- SwissProt: P48507 Human
- SwissProt: O09172 Mouse
- SwissProt: P48508 Rat
- Unigene: 315562 Human
see all -
別名
- Gamma ECS regulatory subunit antibody
- Gamma-ECS regulatory subunit antibody
- Gamma-glutamylcysteine synthetase regulatory subunit antibody
see all
画像
-
![Immunoprecipitation - Anti-GCLM antibody [EPR6668] (ab124827)](http://a.abcam.com/ps/products/124/ab124827/Images/ab124827-266218-ab124827IP1.jpg)
Immunoprecipitation - Anti-GCLM antibody [EPR6668] (ab124827)ab124827 immunoprecipitating GCLM. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/50 and VeriBlot for IP secondary antibody (HRP) (ab131366) at a dilution of 1/10000.
Lane 1: K562 (human chronic myelogenous leukemia) whole cell lysate (10ug)
Lane 2: K562 (human chronic myelogenous leukemia) whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab124827 in K562 (human chronic myelogenous leukemia) whole cell lysate -
![Flow Cytometry - Anti-GCLM antibody [EPR6668] (ab124827)](http://a.abcam.com/ps/products/124/ab124827/Images/ab124827-266217-ab124827FC1.jpg)
Flow Cytometry - Anti-GCLM antibody [EPR6668] (ab124827)ab124827 staining GCLM in HeLa (human cervix adenocarcinoma) cells by flow cytometry. Cells were fixed with 4% paraformaldehyde and the sample was incubated with the primary antibody at a dilution of 1/100. A goat anti rabbit IgG (Alexa Fluor® 488) at a dilution of 1/2000 was used as the secondary antibody.
Isoytype control: Rabbit monoclonal IgG (Black)
Unlabelled control: Cell without incubation with primary antibody and secondary antibody (Blue)
-
![Immunocytochemistry/ Immunofluorescence - Anti-GCLM antibody [EPR6668] (ab124827)](http://a.abcam.com/ps/products/124/ab124827/Images/ab124827-266216-ab124827ICCIF1.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-GCLM antibody [EPR6668] (ab124827)ab124827 staining GCLM in HeLa (human cervix adenocarcinoma) cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 100% methanol. Samples were incubated with primary antibody at a dilution of 1/500. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody. ab7291 (1/1000) and ab150120 (1/1000) were used as counterstains for primary antibody ab124827 and secondary antibody ab150077 respectively. DAPI was used as a nuclear counterstain.
Negative control 1: Rabbit primary antibody and anti-mouse secondary antibody (ab150120)
Negative control 2: Mouse primary antibody (ab7291) and anti-rabbit secondary antibody (ab150077) -
![Western blot - Anti-GCLM antibody [EPR6668] (ab124827)](http://a.abcam.com/ps/products/124/ab124827/Images/ab124827-266214-ab124827WB2.jpg)
Western blot - Anti-GCLM antibody [EPR6668] (ab124827)Anti-GCLM antibody [EPR6668] (ab124827) at 1/10000 dilution + NIH/3T3 (mouse embryo) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution (Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG)
Predicted band size: 31 kDaDiluting and blocking buffer: 5% NFDM /TBST
-
![Immunohistochemistry - Anti-GCLM antibody [EPR6668] (ab124827)](http://a.abcam.com/ps/products/124/ab124827/Images/ab124827-266215-ab124827IHC1.jpg)
Immunohistochemistry - Anti-GCLM antibody [EPR6668] (ab124827)ab124827 staining GCLM in human liver tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250. A goat anti-rabbit IgG H&L (HRP) ab97051 was used as the secondary antibody at 1/500.
Negative control 1: PBS in place of primary antibody.
-
![Western blot - Anti-GCLM antibody [EPR6668] (ab124827)](http://a.abcam.com/ps/products/124/ab124827/Images/ab124827-266213-ab124827WB1.jpg)
Western blot - Anti-GCLM antibody [EPR6668] (ab124827)All lanes : Anti-GCLM antibody [EPR6668] (ab124827) at 1/10000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma) whole cell lysate
Lane 2 : Human skeletal muscle
Lane 3 : Rat kidney tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution
Predicted band size: 31 kDaDiluting and blocking buffer: 5% NFDM/TBST
-
![Western blot - Anti-GCLM antibody [EPR6668] (ab124827)](http://a.abcam.com/ps/products/124/ab124827/Images/GCLM-Primary-antibodies-ab124827-1.jpg)
Western blot - Anti-GCLM antibody [EPR6668] (ab124827)All lanes : Anti-GCLM antibody [EPR6668] (ab124827) at 1/1000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A673 cell lysate
Lane 3 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP conjugated goat anti-rabbit at 1/2000 dilution
Predicted band size: 31 kDa
Observed band size: 30 kDa (why is the actual band size different from the predicted?) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6668] (ab124827)](http://a.abcam.com/ps/products/124/ab124827/Images/GCLM-Primary-antibodies-ab124827-2.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GCLM antibody [EPR6668] (ab124827)ab124827, at 1/100 dilution, staining GCLM in paraffin-embedded Human muscle tissue by Immunohistochemistry. -
](http://a.abcam.com/ps/products/124/ab124827/Images/GCLM-Primary-antibodies-ab124827-3.jpg)
Other-Anti-GCLM antibody [EPR6668](ab124827)
プロトコール
参考文献
This product has been referenced in:
- Dehghan E et al. Hydralazine induces stress resistance and extends C. elegans lifespan by activating the NRF2/SKN-1 signalling pathway. Nat Commun 8:2223 (2017). Read more (PubMed: 29263362) »
- Deng C et al. Low nanomolar concentrations of Cucurbitacin-I induces G2/M phase arrest and apoptosis by perturbing redox homeostasis in gastric cancer cells in vitro and in vivo. Cell Death Dis 7:e2106 (2016). Read more (PubMed: 26890145) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"