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Our Abpromise guarantee covers the use of ab53179 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use at an assay dependent concentration.|
ab53179 staining mouse embryo cells by ICC/IF. Cells were PFA fixed and permeabilized in Triton-X prior to blocking with 5% serum for 1 hr at 25°C. The primary antibody was diluted 1/500 in PBS with 0.5% goat serum and incubated with the sample for 18 hrs at 4°C. A FITC-conjugated goat anti-rabbit was used as the secondary antibody. Note spindle staining in cells undergoing mitosis