製品の概要

  • 製品名Anti-GCET1 antibody [RAM341]
    GCET1 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [RAM341] to GCET1
  • アプリケーション適用あり: ELISA, WB, IP, IHC-Pmore details
    適用なし: IHC-Fr
  • 種交差性
    交差種: Human
  • 免疫原

    Recombinant full length protein (Human)

  • ポジティブ・コントロール
    • Tonsil.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68889 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/100. Detects a band of approximately 46 kDa (predicted molecular weight: 47 kDa).
IP 1/100.
IHC-P 1/100.
  • 追加情報Is unsuitable for IHC-Fr.
  • ターゲット情報

    • 機能Protease inhibitor that inhibits trypsin and trypsin-like serine proteases (in vitro). Inhibits plasmin and thrombin with lower efficiency (in vitro).
    • 組織特異性Highly expressed in normal germinal center (GC) B-cells and GC B-cell-derived malignancies.
    • 配列類似性Belongs to the serpin family.
    • 細胞内局在Cytoplasm; Secreted and Membrane.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Centerin antibody
      • GCET1 antibody
      • Germinal center B cell expressed transcript 1 antibody
      • Germinal center B-cell-expressed transcript 1 protein antibody
      • Seprin A9 antibody
      • Serine proteinase inhibitor A11 antibody
      • Serpin A9 antibody
      • Serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 9 antibody
      • Serpin peptidase inhibitor clade A member 9 antibody
      • SERPINA11 antibody
      • SERPINA11b antibody
      • Serpina9 antibody
      • SPA9_HUMAN antibody
      see all

    Anti-GCET1 antibody [RAM341] 画像

    • Ab68889 staining human GCET1 in Burkitt's Lymphoma (A), Follicular B Cell Lymphoma (B), Diffuse Large B Cell Lymphoma (C) and Lymphocyte Predominant Hodgkin's Lymphoma (D) by immunohistochemistry using formalin fixed, paraffin embedded tissue.

    • Developed using the ECL technique

      Predicted band size : 47 kDa
      Observed band size : 46 kDa (why is the actual band size different from the predicted?)
      Western blot results of GCET1 from transfected cell lines. The first two lanes demonstrate the specificity of ab68889 (1:100) on lysates of transfected HEK-GCET1 cell lines. A 46kDa band specific to the GCET1 protein was observed in GC-DLBCL (SUDHL4 and SUDHL6) and some other DLBCL (KARPAS 422, DB and HT). The GCET1 protein was not present in a subset of DLBCL (MHHPREB1, DOHH2).

      The top bands show the GCET1 protein, whereas the bottom bands are GAPDH, used as a reference.
    • Ab68889 staining human GCET1 in human tonsil by immunohistochemistry using paraffin embedded tissue.

    Anti-GCET1 antibody [RAM341] (ab68889) 使用論文

    This product has been referenced in:
    • Lu TX  et al. Epstein-Barr virus positive diffuse large B-cell lymphoma predict poor outcome, regardless of the age. Sci Rep 5:12168 (2015). IHC ; Human . Read more (PubMed: 26202875) »
    • Xie Y  et al. p53 expression is a strong marker of inferior survival in de novo diffuse large B-cell lymphoma and may have enhanced negative effect with MYC coexpression: a single institutional clinicopathologic study. Am J Clin Pathol 141:593-604 (2014). Human . Read more (PubMed: 24619762) »

    See all 6 Publications for this product

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    Both of our GCET1 antibodies, Ab68889 and Ab86673, are research use only antibodies and are not approved for diagnostic usage.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"