Anti-GCET1 抗体 [RAM341] (ab68889)
Key features and details
- Mouse monoclonal [RAM341] to GCET1
- Suitable for: IHC-P
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-GCET1 antibody [RAM341]
GCET1 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [RAM341] to GCET1 -
由来種
Mouse -
アプリケーション
適用あり: IHC-Pmore details
適用なし: IHC-Fr -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human GCET1.
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ポジティブ・コントロール
- IHC-P: Human Burkitt's Lymphoma, Follicular B Cell Lymphoma, Diffuse Large B Cell Lymphoma and Lymphocyte Predominant Hodgkin's Lymphoma tissue. Human tonsil tissue.
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
This antibody was purified using a AKTA prime. -
ポリ/モノ
モノクローナル -
クローン名
RAM341 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab68889の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100.
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特記事項 |
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IHC-P
1/100. |
ターゲット情報
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機能
Protease inhibitor that inhibits trypsin and trypsin-like serine proteases (in vitro). Inhibits plasmin and thrombin with lower efficiency (in vitro). -
組織特異性
Highly expressed in normal germinal center (GC) B-cells and GC B-cell-derived malignancies. -
配列類似性
Belongs to the serpin family. -
細胞内局在
Cytoplasm; Secreted and Membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 327657 Human
- SwissProt: Q86WD7 Human
- Unigene: 317970 Human
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別名
- Centerin antibody
- GCET1 antibody
- Germinal center B cell expressed transcript 1 antibody
see all
画像
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Ab68889 staining human GCET1 in Burkitt's Lymphoma (A), Follicular B Cell Lymphoma (B), Diffuse Large B Cell Lymphoma (C) and Lymphocyte Predominant Hodgkin's Lymphoma (D) by immunohistochemistry using formalin fixed, paraffin embedded tissue.
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IHC image of GCET1 staining in human tonsil formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab68889, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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Ab68889 staining human GCET1 in human tonsil by immunohistochemistry using paraffin embedded tissue.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab68889 は 10 報の論文で使用されています。
- Perfecto-Avalos Y et al. Discriminant analysis and machine learning approach for evaluating and improving the performance of immunohistochemical algorithms for COO classification of DLBCL. J Transl Med 17:198 (2019). PubMed: 31185999
- Tessier-Cloutier B et al. Cell of origin in diffuse large B-cell lymphoma in systemic lupus erythematosus: molecular and clinical factors associated with survival. Lupus Sci Med 6:e000324 (2019). PubMed: 31205728
- Boltežar L et al. Comparison of the algorithms classifying the ABC and GCB subtypes in diffuse large B-cell lymphoma. Oncol Lett 15:6903-6912 (2018). PubMed: 29731865
- Lu TX et al. Epstein-Barr virus positive diffuse large B-cell lymphoma predict poor outcome, regardless of the age. Sci Rep 5:12168 (2015). IHC ; Human . PubMed: 26202875
- Tzankov A et al. Multiparameter analysis of homogeneously R-CHOP-treated diffuse large B cell lymphomas identifies CD5 and FOXP1 as relevant prognostic biomarkers: report of the prospective SAKK 38/07 study. J Hematol Oncol 8:70 (2015). IHC . PubMed: 26071053
- Xie Y et al. p53 expression is a strong marker of inferior survival in de novo diffuse large B-cell lymphoma and may have enhanced negative effect with MYC coexpression: a single institutional clinicopathologic study. Am J Clin Pathol 141:593-604 (2014). Human . PubMed: 24619762
- Tan LH & Tan SY Aberrant immunoarchitecture distinguishes hyperplastic germinal centres in pattern 1 angioimmunoblastic T-cell lymphoma from reactive follicles. Hematol Oncol 32:145-54 (2014). Human . PubMed: 24254640
- Rossi D et al. Molecular history of Richter syndrome: origin from a cell already present at the time of chronic lymphocytic leukemia diagnosis. Int J Cancer 130:3006-10 (2012). PubMed: 21796624
- Sebestyén A et al. Activity and complexes of mTOR in diffuse large B-cell lymphomas-a tissue microarray study. Mod Pathol : (2012). PubMed: 22899290
- Montes-Moreno S et al. Gcet1 (centerin), a highly restricted marker for a subset of germinal center-derived lymphomas. Blood 111:351-8 (2008). PubMed: 17898315