製品の概要

  • 製品名Anti-GCDFP 15 antibody [EP1582Y]
    GCDFP 15 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP1582Y] to GCDFP 15
  • アプリケーション適用あり: WB, IHC-P, ICC, Flow Cytmore details
    適用なし: IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide corresponding to residues near the N-terminus of human GCDFP 15

  • ポジティブ・コントロール
    • T47D cell lysate, human breast carcinoma tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab62363 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Predicted molecular weight: 17 kDa.Can be blocked with GCDFP 15 peptide (ab155648).
IHC-P 1/100 - 1/250.
ICC 1/50 - 1/100.
Flow Cyt 1/20. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for IP.
  • ターゲット情報

    • 組織特異性Expressed in pathological conditions of the mammary gland and in several exocrine tissues, such as the lacrimal, salivary, and sweat glands.
    • 配列類似性Belongs to the PIP family.
    • 細胞内局在Secreted.
    • Information by UniProt
    • 参照データベース
    • 別名
      • BRST-2 antibody
      • BRST2 antibody
      • GCDFP-15 antibody
      • GCDGP15 antibody
      • gp17 antibody
      • GPIP4 antibody
      • Gross cystic disease fluid protein 15 antibody
      • PIP antibody
      • PIP_HUMAN antibody
      • Prolactin induced protein antibody
      • Prolactin inducible protein precursor antibody
      • Prolactin-induced protein antibody
      • Prolactin-inducible protein antibody
      • SABP antibody
      • Secretory actin binding protein antibody
      • Secretory actin-binding protein antibody
      see all

    Anti-GCDFP 15 antibody [EP1582Y] 画像

    • Anti-GCDFP 15 antibody [EP1582Y] (ab62363) at 1/2000 dilution + T47D cell lysate at 10 µg

      Secondary
      goat anti rabbit HRP at 1/2000 dilution

      Predicted band size : 17 kDa
      Observed band size : 17 kDa
    • Immunocytochemistry/Immunofluorescence analysis of MCF-7 (human breast carcinoma) cells labelling GCDFP 15 with ab62363 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton-X. ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab7291 Anti-Tubulin antibody (1/1000) followed by ab150120   AlexaFluor®594 Goat anti-Mouse secondary (1/1000). Nuclei were counterstained with DAPI (blue).

      For negative control 1, ab62363 at dilution of 1/100 was used followed by anti-mouse secondary antibody (ab150120) at 1/1000 dilution. For negative control 2, ab7291; 1/1000 was used followed by anti-rabbit secondary antibody (ab150077) at 1/1000 dilution.  

      Confocal image showing cytoplasmic staining on MCF7 cells.

    • Immunohistochemical analysis of GCDFP 15 expression in paraffin embedded human breast carcinoma using 1/100-1/250 dilution of ab62363
    • ab62363 showing positive staining in Cystic breast disease tissue.

    • Flow cytometry analysis of T47D (human mammary gland ductal carcinoma) (pink) with ab62363 at dilution of 1/20. The secondary antibody used was goat anti rabbit IgG (FITC) at dilution of 1/150. Cells were fixed with 2% paraformaldehyde. Isotype control antibody used was Rabbit monoclonal IgG (green).

    • ab62363 showing positive staining in Salivary gland tissue.

    • ab62363 showing negative staining in Hepatocellular carcinoma tissue.

    • ab62363 showing negative staining in Prostate hyperplasia tissue.

    Anti-GCDFP 15 antibody [EP1582Y] (ab62363) 使用論文

    This product has been referenced in:
    • Naderi A & Meyer M Prolactin-induced protein mediates cell invasion and regulates integrin signaling in estrogen receptor-negative breast cancer. Breast Cancer Res 14:R111 (2012). WB, IHC-P ; Human . Read more (PubMed: 22817771) »
    • Baniwal SK  et al. Runx2 transcriptome of prostate cancer cells: insights into invasiveness and bone metastasis. Mol Cancer 9:258 (2010). WB ; Human . Read more (PubMed: 20863401) »

    See all 2 Publications for this product

    Product Wall

    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (HeLa)
    Specification HeLa
    Permeabilization No
    Fixative Methanol
    Username

    Dr. Kirk McManus

    Verified customer

    投稿 Jan 28 2014

    The antibody ab62363 has an immunogen near the N-terminus of human GCDFP 15. The peptide ab48069 you mention however is derived from the internal region of GCDFP 15 and therefore ab62363 will not bind to it.

    I have been in contact with the la...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"