Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
Soluble in water to 25 mM (with heating) and in DMSO to 100 mM (with heating)
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
ab65783 staining NR2B in SKNSH cells treated with GBR 12909 dihydrochloride (ab120607), by ICC/IF. Decrease in NR2B expression correlates with increased concentration of GBR 12909 dihydrochloride, as described in literature. The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120607 (GBR 12909 dihydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab65783 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
This product has been referenced in:
Wiskerke J et al. µ-Opioid receptors in the nucleus accumbens shell region mediate the effects of amphetamine on inhibitory control but not impulsive choice. J Neurosci31:262-72 (2011).
Read more (PubMed: 21209211) »
Szasz BK et al. Nicotinic acetylcholine receptor antagonistic property of the selective dopamine uptake inhibitor, GBR-12909 in rat hippocampal slices. Neuroscience145:344-9 (2007).
Read more (PubMed: 17207584) »
Mike A et al. Inhibitory effect of the DA uptake blocker GBR 12909 on sodium channels of hippocampal neurons. Neuroreport14:1945-9 (2003).
Read more (PubMed: 14561926) »