GBR 12909 dihydrochloride, Dopamine transport inhibitor (ab120607)
Key features and details
- Selective dopamine transport inhibitor
- CAS Number: 67469-78-7
- Purity: > 98%
- Soluble in water to 25 mM (with heating) and in DMSO to 100 mM (with heating)
- Form / State: Solid
- Source: Synthetic
製品の概要
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製品名
GBR 12909 dihydrochloride, Dopamine transport inhibitor -
製品の詳細
Selective dopamine transport inhibitor -
生理活性の詳細
Potent, selective dopamine reuptake inhibitor (IC50 = 2.32 µM in rat hippocampal slices; IC50 = 6-35 µM in whole cell patch clamp recordings).
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精製度
> 98% -
CAS 番号
67469-78-7 -
構造式
製品の特性
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体系名
1-[2-[Bis-(4-fluorophenyl)methoxy]ethyl]-4-(3-phenylpropyl)piperazine dihydrochloride -
分子量
523.49 -
分子式
C28H32F2N2O.2HCl -
PubChem 登録番号
104920 -
保存方法
Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months. -
溶解性
Soluble in water to 25 mM (with heating) and in DMSO to 100 mM (with heating) -
使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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SMILES 線形表記
C1CN(CCN1CCCC2=CC=CC=C2)CCOC(C3=CC=C(C=C3)F)C4=CC=C(C=C4)F.Cl.Cl -
由来
Synthetic
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研究分野
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab120607の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Functional Studies |
Use at an assay dependent concentration.
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特記事項 |
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Functional Studies
Use at an assay dependent concentration. |
画像
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2D chemical structure image of ab120607, GBR 12909 dihydrochloride, Dopamine transport inhibitor
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ab65783 staining NR2B in SKNSH cells treated with GBR 12909 dihydrochloride (ab120607), by ICC/IF. Decrease in NR2B expression correlates with increased concentration of GBR 12909 dihydrochloride, as described in literature.
The cells were incubated at 37°C for 10 minutes in media containing different concentrations of ab120607 (GBR 12909 dihydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab65783 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
参考文献 (0)
ab120607 は論文での使用が確認できていません。