製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GBP2
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Rat, Rabbit, Horse, Guinea pig, Cow, Cat, Pig, African green monkey
  • 免疫原

    Synthetic peptide: KSHTKGIWMW CVPHPKKPEH TLVLLDTEGL GDIEKGDNEN DSWIFALAIL, corresponding to a region within N terminal amino acids 72 - 121 of Human GBP2 (NP_004111).

  • ポジティブ・コントロール
    • 721_B cell lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab81486 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 67 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ELISA Use at an assay dependent concentration.

ELISA titre using peptide based assay: 1/312500.

ターゲット情報

  • 関連性
    Guanylate-binding proteins (GBPs) are characterized by their ability to specifically bind guanine nucleotides (GMP, GDP, and GTP). GBP2 is a GTPase that converts GTP to GDP and GMP.
  • 細胞内局在
    Cell membrane; Lipid-anchor; Cytoplasmic side
  • 参照データベース
  • 別名
    • GBP 2 antibody
    • GTP binding protein 2 antibody
    • Guanine nucleotide binding protein 2 antibody
    • Guanylate binding protein 2 interferon inducible antibody
    • HuGBP 2 antibody
    • HuGBP2 antibody
    • Interferon induced guanylate binding protein 2 antibody
    see all

画像

  • Anti-GBP2 antibody (ab81486) at 1 µg/ml (in 5% skim milk / PBS buffer) + 721_B cell lysate at 10 µg

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 67 kDa
    Observed band size : 65 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 30 kDa,32 kDa,40 kDa. We are unsure as to the identity of these extra bands.

プロトコール

参考文献

ab81486 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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