製品の概要

  • 製品名Anti-GBF1 antibody
    GBF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GBF1
  • アプリケーション適用あり: WB, IP, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Guinea pig, Chimpanzee, Rhesus monkey, Chinese Hamster, Orangutan
  • 免疫原

    A region between residue 1809 and 1859 of Human GBF1 (NP_004184.1).

  • ポジティブ・コントロール
    • Whole cell lysate from HeLa, 293T and NIH3T3 cells

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/2000 - 1/10000. Predicted molecular weight: 206 kDa.
IP Use at 2-5 µg/mg of lysate.
ICC/IF Use a concentration of 1 µg/ml.
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

ターゲット情報

  • 機能Guanine-nucleotide exchange factor (GEF) for members of the Arf family of small GTPases involved in trafficking in the early secretory pathway; its GEF activity initiates the coating of nascent vesicles via the localized generation of activated ARFs through replacement of GDP with GTP. Recruitment to cis-Golgi membranes requires membrane association of Arf-GDP and can be regulated by ARF1, ARF3, ARF4 and ARF5. Involved in the recruitment of the COPI coat complex to the endoplasmic reticulum exit sites (ERES), and the endoplasmic reticulum-Golgi intermediate (ERGIC) and cis-Golgi compartments which implicates ARF1 activation. Involved in COPI vesicle-dependent retrograde transport from the ERGIC and cis-Golgi compartments to the endoplasmatic reticulum (ER) (PubMed:16926190, PubMed:17956946, PubMed:18003980, PubMed:12047556, PubMed:12808027, PubMed:19039328, PubMed:24213530). Involved in the trans-Golgi network recruitment of GGA1, GGA2, GGA3, BIG1, BIG2, and the AP-1 adaptor protein complex related to chlathrin-dependent transport; the function requires its GEF activity (probably at least in part on ARF4 and ARF5) (PubMed:23386609). Has GEF activity towards ARF1 (PubMed:15616190). Has in vitro GEF activity towards ARF5 (By similarity). Involved in the processing of PSAP (PubMed:17666033). Required for the assembly of the Golgi apparatus (PubMed:12808027, PubMed:18003980). The AMPK-phosphorylated form is involved in Golgi disassembly during mitotis and under stress conditions (PubMed:18063581, PubMed:23418352). May be involved in the COPI vesicle-dependent recruitment of PNPLA2 to lipid droplets; however, this function is under debate (PubMed:19461073, PubMed:22185782). In neutrophils, involved in G protein-coupled receptor (GPCR)-mediated chemotaxis und superoxide production. Proposed to be recruited by phosphatidylinositol-phosphates generated upon GPCR stimulation to the leading edge where it recruits and activates ARF1, and is involved in recruitment of GIT2 and the NADPH oxidase complex (PubMed:22573891).
  • 組織特異性Ubiquitous.
  • 配列類似性Contains 1 SEC7 domain.
  • ドメインThe DCB (dimerization and cyclophiln-binding) and HUS (homology upstream of Sec7) domains are necessary for dimerization. The DCB domain is proposed to support constitutive homodimerization; the HUS domain interacts with the DCB domain which may occur intramolecular or intermolecuar.
  • 翻訳後修飾AMPK-mediated phosphorylation at Thr-1337 is induced by 2-deoxyglucose (2-DG) and AICA ribonucleotide, and occurs during mitosis leading to membrane disassociation and inactivation of ARF1 during mitosis.
  • 細胞内局在Golgi apparatus, cis-Golgi network. Endoplasmic reticulum-Golgi intermediate compartment. Golgi apparatus, trans-Golgi network. Cytoplasm. Lipid droplet. Membrane. Cycles rapidly on and off early Golgi membranes (PubMed:15616190). Stabilized on membranes when complexed with ARF1-GDP and is released from both ARF1 and membranes after it catalyzes GDP displacement and ARF1 binds GTP. Continuous cycles of recruitment and dissociation of GBF1 to membranes are required for sustained ARF activation and COP I recruitment (PubMed:15813748). In neutrophils is translocated from the Golgi to the leading edge upon GPCR stimulation (PubMed:22573891). Localization to lipid droplets is questionable (PubMed:22185782).
  • Information by UniProt
  • 参照データベース
  • 別名
    • ARF1GEF antibody
    • BFA resistant GEF 1 antibody
    • BFA-resistant GEF 1 antibody
    • FLJ21263 antibody
    • FLJ21500 antibody
    • GBF1 antibody
    • GBF1_HUMAN antibody
    • golgi brefeldin A resistant guanine nucleotide exchange factor 1 antibody
    • Golgi specific brefeldin A resistance factor 1 antibody
    • Golgi specific Brefeldin A-resistance factor antibody
    • Golgi-specific brefeldin A-resistance guanine nucleotide exchange antibody
    • Golgi-specific brefeldin A-resistance guanine nucleotide exchange factor 1 antibody
    • KIAA0248 antibody
    • MGC134877 antibody
    • MGC134878 antibody
    see all

Anti-GBF1 antibody 画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling GBF1 with ab86071 at 1/200 (1µg/ml). Detection: DAB.
  • All lanes : Anti-GBF1 antibody (ab86071) at 0.04 µg/ml

    Lane 1 : HeLa whole cell lysate at 50 µg
    Lane 2 : HeLa whole cell lysate at 15 µg
    Lane 3 : HeLa whole cell lysate at 5 µg
    Lane 4 : 293T cell lysate at 50 µg
    Lane 5 : mouse NIH3T3 cell lysate at 50 µg

    Developed using the ECL technique

    Predicted band size : 206 kDa


    Exposure time : 10 seconds
  • Immunoprecipitation/ Western Blot of ab86071. Lane 1: ab86071 at 3µg/mg whole cell lysate. Lane 2: Control IgG. ab86071 at 1µg/ml for WB. Whole cell lysate from Hela cells at 1mg for IP, 20% of IP loaded. Chemiluminescence with an exposure time of 3 seconds.
  • ab86071 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab86071 at 1µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab86071 staining GBF1 in HeLa cells treated with Exo-1 (ab120292), by ICC/IF. Increase in GBF1 expression correlates with increased concentration of Exo-1 as described in literature.
    The cells were incubated at 37°C for 5 minutes in media containing different concentrations of ab120292 ( Exo-1) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab86071 (5 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

Anti-GBF1 antibody (ab86071) 使用論文

This product has been referenced in:
  • Richards AL  et al. Generation of unique poliovirus RNA replication organelles. MBio 5:e00833-13 (2014). ICC/IF ; Human . Read more (PubMed: 24570367) »
  • Covington JD  et al. Skeletal muscle perilipin 3 and coatomer proteins are increased following exercise and are associated with fat oxidation. PLoS One 9:e91675 (2014). WB ; Human . Read more (PubMed: 24632837) »

See all 4 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (DU145)
Permeabilization Yes - 0.2% Triton X-100
Specification DU145
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative Formaldehyde
Username

Dr. Armen Petrosyan

Verified customer

投稿 Oct 31 2016

Thank you for contacting us. We have two antibodies in our catalogue against GBF1. Both are rabbit polyclonals, ab86071 and ab105111. ab86071 has been tested with human and mouse samples with immunoprecipitation and Western blotting. ab105111 a...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"