製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to GBA2
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    GBA2 (NP_065995.1, 1 a.a. ~ 927 a.a) full-length human protein.

  • ポジティブ・コントロール
    • GBA2 transfected 293T lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab69366 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 105 kDa (predicted molecular weight: 105 kDa).

ターゲット情報

  • 関連性
    GBA2 is a non-lysosomal glucosylceramidase that catalyzes the conversion of glucosylceramide to free glucose and ceramide. It is involved in sphingomyelin generation and prevention of glycolipid accumulation. GBA2 may also catalyze the hydrolysis of bile acid 3-O-glucosides, however, the relevance of such activity is unclear in vivo.
  • 細胞内局在
    Cell membrane; Single-pass membrane protein. Note=Not localized to lipid rafts.
  • 参照データベース
  • 別名
    • AD035 antibody
    • Beta glucocerebrosidase 2 antibody
    • Beta glucosidase 2 antibody
    • DKFZp762K054 antibody
    • glucosidase, beta (bile acid) 2 antibody
    • Glucosylceramidase 2 antibody
    • KIAA1605 antibody
    • MGC16895 antibody
    • NLGase antibody
    • Non lysosomal glucosylceramidase antibody
    • SPG46 antibody
    see all

画像

  • All lanes : Anti-GBA2 antibody (ab69366) at 1/500 dilution

    Lane 1 : GBA2 transfected 293T lysate
    Lane 2 : Non-transfected 293T lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

    Predicted band size : 105 kDa
    Observed band size : 105 kDa

プロトコール

参考文献

ab69366 has not yet been referenced specifically in any publications.

レビューと Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Neuroblastoma cell line Neuro2a)
Permeabilization
Yes - 0.1% PBS-Tween
Specification
Neuroblastoma cell line Neuro2a
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Massimo Aureli

Verified customer

投稿 Apr 29 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Bronchial epithelial cells overexpressing GBA2)
Permeabilization
Yes - 0.1% PBS-Tween
Specification
Bronchial epithelial cells overexpressing GBA2
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Massimo Aureli

Verified customer

投稿 Apr 25 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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