製品の概要

  • 製品名Anti-Gata6 antibody
    Gata6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Gata6
  • アプリケーション適用あり: WBmore details
  • 種交差性

    交差が予測される動物種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide derived from within residues 250 - 350 of Human Gata6.

    (Peptide available as ab24411.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human heart tissue lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab39123 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 45 kDa).

ターゲット情報

Anti-Gata6 antibody 画像

  • Anti-Gata6 antibody (ab39123) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Predicted band size : 45 kDa
    Observed band size : 45 kDa
    Additional bands at : 30 kDa,60 kDa (possible isoform),70 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-Gata6 antibody (ab39123) 使用論文

This product has been referenced in:
  • Kee HJ  et al. HDAC inhibition suppresses cardiac hypertrophy and fibrosis in DOCA-salt hypertensive rats via regulation of HDAC6/HDAC8 enzyme activity. Kidney Blood Press Res 37:229-39 (2013). Rat . Read more (PubMed: 23868068) »
  • Barbuti A  et al. Mesoangioblasts from ventricular vessels can differentiate in vitro into cardiac myocytes with sinoatrial-like properties. J Mol Cell Cardiol 48:415-23 (2010). Read more (PubMed: 19837079) »

See all 2 Publications for this product

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Flow Cytometry
Sample Mouse Cell (differentiated mouse ES cell (D3 line) day 14)
Specification differentiated mouse ES cell (D3 line) day 14
Preparation Cell harvesting/tissue preparation method: trypsinisation of EBs to single cell suspension
Sample buffer: FACS buffer (0.2%BSA, 0.1%sodium azide in PBS)
Fixation Paraformaldehyde
Permeabilization Yes - 70% methanol
Gating Strategy FSC vs SSC on negative control sample
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Dr. Lisa Mohamet

Verified customer

投稿 Jun 04 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Cell lysate - other (differentiated mouse EBs (day14))
Loading amount 20 µg
Specification differentiated mouse EBs (day14)
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: rt°C
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Abcam user community

Verified customer

投稿 Jun 04 2010

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (Differentiated embryonic stem cells, feeder free)
Specification Differentiated embryonic stem cells, feeder free
Fixative Paraformaldehyde
Permeabilization Yes - see blocking buffer below
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: RT°C
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Abcam user community

Verified customer

投稿 Aug 12 2009

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cell (Differentiated mouse ES cell (D3 line))
Specification Differentiated mouse ES cell (D3 line)
Fixative Paraformaldehyde
Permeabilization Yes - see blocking buffer below
Blocking step 0.1%BSA, 1%serum, 0.1% triton in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Username

Abcam user community

Verified customer

投稿 Aug 12 2009

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"