製品の概要

  • 製品名
    Anti-GATA5 antibody
  • 製品の詳細
    Rabbit polyclonal to GATA5
  • 特異性
    This antibody recognizes GATA5
  • アプリケーション
    適用あり: ELISA, WB, ICC/IFmore details
  • 種交差性
    交差種: Mouse
    交差が予測される動物種: Rat, Human
  • 免疫原

    A region within synthetic peptide: LNSESSATTL KAESSLASPV CAGPTITSQA SSPADESLAS SHLEFKFEPE, corresponding to C terminal amino acids 325-374 of Human GATA5

  • ポジティブ・コントロール
    • SP2/0 lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 2% Sucrose, PBS
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab48820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1.5625e+006.
WB Use a concentration of 1.25 µg/ml. Predicted molecular weight: 44 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
ICC/IF 1/100.

ターゲット情報

画像

  • Anti-GATA5 antibody (ab48820) at 1.25 µg/ml + SP2/0 lysate at 10 µg with 5% skimmed milk in PBS.

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 41 kDa (why is the actual band size different from the predicted?)
    Gel concentration 12%
  • ab48820 staining GATA5 in murine epicardial explant culture by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in formaldehyde, permeabilized using 0.5% Triton X-100 in PBS, blocked with 1% goat serum 10% BSA 0.1% Triton X-100 PBS and then incubated with ab48820 at a 1/100 dilution for 16 hours at 4°C. The secondary used was an Alexa-Fluor 594 conjugated goat anti-rabbit IgG (H+L) used at a 1/200 dilution.

    See Abreview

参考文献

This product has been referenced in:
  • Risebro CA  et al. Characterisation of the human embryonic and foetal epicardium during heart development. Development 142:3630-6 (2015). IHC ; Human . Read more (PubMed: 26395486) »

See 1 Publication for this product

レビューと Q&A

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (epicardial explant culture)
Specification
epicardial explant culture
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Tx100 in PBS
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 20°C
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投稿 Jun 19 2012

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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