製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GATA4
  • アプリケーション
    適用あり: IHC-P, ICC/IF, IHC-Fr, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human, Rhesus monkey
    交差が予測される動物種: Dog, Pig
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 100 - 200 of Human GATA4.

    (Peptide available as ab94555.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HepG2; HEK293; Caco 2.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab84593 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.
IHC-Fr 1/50.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa).

ターゲット情報

  • 機能
    Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
  • 関連疾患
    Atrial septal defect 2
    Ventricular septal defect 1
    Tetralogy of Fallot
    Atrioventricular septal defect 4
    Testicular anomalies with or without congenital heart disease
    GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • 配列類似性
    Contains 2 GATA-type zinc fingers.
  • 翻訳後修飾
    Methylation at Lys-300 attenuates transcriptional activity.
  • 細胞内局在
    Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ASD2 antibody
    • GATA 4 antibody
    • GATA binding protein 4 antibody
    • GATA-binding factor 4 antibody
    • gata4 antibody
    • GATA4_HUMAN antibody
    • MGC126629 antibody
    • Transcription factor GATA 4 antibody
    • Transcription factor GATA-4 antibody
    • Transcription factor GATA4 antibody
    • VSD1 antibody
    see all

Anti-GATA4 antibody 画像

  • All lanes : Anti-GATA4 antibody (ab84593) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 12 minutes
  • ab84593 staining GATA4 (green) in mouse embryo tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde, permeabilized in 1% Triton X-100 and blocked with 10% goat serum for 1 hour at room temperature; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/50 in 10% goat serum) for 16 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/400) was used as the secondary antibody. Red - Myh6/7, Blue - DAPI.

    See Abreview

  • Immunofluorescence image of Gata4 (ab84593) on human embryonic stem cell derived cardiomyocytes. The cells were fixed in methanol and permeabilized in 0.1% Triton-X-100. In this image you can see a cardiomyocyte stained in red with a cardiac alpha actinin antibody and both the cardiomyocyte and a fibroblast stained in green with ab84593.

    See Abreview

  • All lanes : Anti-GATA4 antibody (ab84593) at 1 µg/ml

    Lane 1 : Human heart tissue lysate - total protein (ab29431)
    Lane 2 : Human Fetal Heart - Total Protein Tissue Lysate
    Lane 3 : Heart (Mouse) Tissue Lysate
    Lane 4 : Heart (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
    Additional bands at : 110 kDa. We are unsure as to the identity of these extra bands.

Anti-GATA4 antibody (ab84593) 使用論文

This product has been referenced in:
  • Jiang XY  et al. Inhibition of Gata4 and Tbx5 by Nicotine-Mediated DNA Methylation in Myocardial Differentiation. Stem Cell Reports 8:290-304 (2017). WB, IF, Flow Cyt . Read more (PubMed: 28111280) »
  • Kannappan R  et al. p53 Modulates the Fate of Cardiac Progenitor Cells Ex Vivo and in the Diabetic Heart In Vivo. EBioMedicine 16:224-237 (2017). Read more (PubMed: 28163043) »

See all 11 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Pig Cell (Paraffin embedded adult pig heart)
Permeabilization
Yes - 1% Triton X-100, 30' RT
Specification
Paraffin embedded adult pig heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 13 2017

Application
Western blot
Sample
Pig Tissue lysate - whole (Total heart)
Gel Running Conditions
Reduced Denaturing (12%)
Loading amount
20 µg
Specification
Total heart
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 13 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Intestinal epithelium)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate Buffer
Permeabilization
Yes - Triton in TBS
Specification
Intestinal epithelium
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jul 11 2017

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (heart)
Specification
heart
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 4°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 13 2015

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (Cardiomyocytes)
Specification
Cardiomyocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Username

Abcam user community

Verified customer

投稿 Feb 11 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification
Human pluripotent stem cell derived cardiomyocyte
Permeabilization
Yes - saponin
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Sep 19 2014

Application
Immunohistochemistry (Frozen sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Sample
Mouse Tissue sections (mouse embryo)
Specification
mouse embryo
Permeabilization
Yes - 1% triton x-100
Fixative
Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 03 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: rt°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrition buffer
Sample
Mouse Tissue sections (mouse embryo)
Specification
mouse embryo
Permeabilization
Yes - 1% triton x-100
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Jan 02 2014

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12)
Sample
Mouse Cell lysate - nuclear (Gata4 over-expressing undifferentiated mouse ES ce)
Specification
Gata4 over-expressing undifferentiated mouse ES ce
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Ms. Elisa Ponsele

Verified customer

投稿 Dec 20 2013

Thank you for contacting us. Your credit note ID is xxxxxx. I am sorry that this antibody did not perform as stated on the datasheet. I have asked our accounting department to issue a credit note for you, which can be redeemed against the invoice...

Read More

1-10 of 18 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録