製品の概要

  • 製品名Anti-GATA4 antibody
    GATA4 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GATA4
  • アプリケーション適用あり: WB, ELISA, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Rat, Human
  • 免疫原

    Synthetic unphosphorylates peptide derived from human GATA4 around the phosphorylation site of serine 105 (P-V-SP-P-R).

  • ポジティブ・コントロール
    • HeLa cell extracts and HeLa cells.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab61767 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
ELISA 1/20000.
IHC-P Use a concentration of 2 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF 1/500 - 1/1000.

ターゲット情報

  • 機能Transcriptional activator that binds to the consensus sequence 5'-AGATAG-3' and plays a key role in cardiac development (PubMed:24000169). Involved in bone morphogenetic protein (BMP)-mediated induction of cardiac-specific gene expression (By similarity). Binds to BMP response element (BMPRE) DNA sequences within cardiac activating regions (By similarity). Acts as a transcriptional activator of ANF in cooperation with NKX2-5 (By similarity). Promotes cardiac myocyte enlargement (PubMed:20081228). Required during testicular development (PubMed:21220346). May play a role in sphingolipid signaling by regulating the expression of sphingosine-1-phosphate degrading enzyme, spingosine-1-phosphate lyase (PubMed:15734735).
  • 関連疾患Atrial septal defect 2
    Ventricular septal defect 1
    Tetralogy of Fallot
    Atrioventricular septal defect 4
    Testicular anomalies with or without congenital heart disease
    GATA4 mutations can predispose to dilated cardiomyopathy (CMD), a disorder characterized by ventricular dilation and impaired systolic function, resulting in congestive heart failure and arrhythmia. Patients are at risk of premature death.
  • 配列類似性Contains 2 GATA-type zinc fingers.
  • 翻訳後修飾Methylation at Lys-300 attenuates transcriptional activity.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ASD2 antibody
    • GATA 4 antibody
    • GATA binding protein 4 antibody
    • GATA-binding factor 4 antibody
    • gata4 antibody
    • GATA4_HUMAN antibody
    • MGC126629 antibody
    • Transcription factor GATA 4 antibody
    • Transcription factor GATA-4 antibody
    • Transcription factor GATA4 antibody
    • VSD1 antibody
    see all

Anti-GATA4 antibody 画像

  • All lanes : Anti-GATA4 antibody (ab61767) at 1/500 dilution

    Lane 1 : HeLa cell extracts with no immunising peptide
    Lane 2 : HeLa cell extracts with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.


    Predicted band size : 46 kDa
    Observed band size : 46 kDa
  • Immunofluorescence analysis of HeLa cells, using ab61767 at a dilution of 1/500 - 1/1000, with and without the immunising peptide.
  • ab61767 (2µg/ml) staining GATA4 in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is weak nuclear staining.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-GATA4 antibody (ab61767) 使用論文

This product has been referenced in:
  • Elabd C  et al. DNA methyltransferase-3-dependent nonrandom template segregation in differentiating embryonic stem cells. J Cell Biol 203:73-85 (2013). Read more (PubMed: 24127215) »
  • He Z  et al. Transduction of Wnt11 promotes mesenchymal stem cell transdifferentiation into cardiac phenotypes. Stem Cells Dev 20:1771-8 (2011). WB ; Rat . Read more (PubMed: 21231807) »

See all 6 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample Human Cell (Human pluripotent stem cell derived cardiomyocytes)
Specification Human pluripotent stem cell derived cardiomyocytes
Permeabilization Yes - saponin
Fixative Paraformaldehyde
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Verified customer

投稿 Aug 28 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Monkey Tissue sections (Testis)
Specification Testis
Fixative Formaldehyde
Permeabilization No
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
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投稿 Aug 09 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Rat Cell (cardiac cell)
Specification cardiac cell
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% TritonX
Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 37°C
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Verified customer

投稿 Jul 08 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"