Mouse monoclonal [mAbcam77073] to GATA3
Mouse, Rat, Chicken, Cow, Pig
Synthetic peptide derived from within residues 1 - 100 of Human GATA3.
(Peptide available as
This antibody gave a positive signal in the following human tissue lysates: Thymus; Lymph Node; Spleen; Tonsil.
This antibody clone is manufactured by Abcam.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact
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Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium Azide
Constituents: PBS, pH 7.4
Concentration information loading...
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in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use at an assay dependent concentration. Detects a band of approximately 55 kDa (predicted molecular weight: 48 kDa).
Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
T-cells and endothelial cells.
Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
Contains 2 GATA-type zinc fingers.
Information by UniProt
There are 2 isoforms produced by alternative splicing.
GATA 3 antibody
GATA binding factor 3 antibody
GATA binding protein 3 antibody
Western blot - GATA3 antibody [mAbcam77073] (ab77073)
All lanes :
Anti-GATA3 antibody [mAbcam77073] (ab77073) at 5 µg/ml
Lane 1 :
Human thymus tissue lysate - total protein (
Lane 2 :
Human lymph node tissue lysate - total protein (
Lane 3 :
Human spleen tissue lysate - total protein (
Lane 4 :
Tonsil (Human) Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary All lanes :
Goat Anti-Mouse IgG H&L (HRP) preadsorbed (
) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size:
Observed band size:
55 kDa (
why is the actual band size different from the predicted?
Additional bands at:
90 kDa. We are unsure as to the identity of these extra bands.
The binding pattern of ab77073 in human thymus, lymph node, spleen and tonsil tissue lysates is similar to that of the polyclonal antibody to the same target (
) in MOLT4, Jurkat, THP1 and Ramos whole cell lysates.
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"