製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GATA3
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    3 synthetic peptides (human) conjugated to KLH

  • ポジティブ・コントロール
    • Recombinant GATA3 protein expressed in induced culture of E.coli (one shot Top10 competent cells).

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報
    ELISA: Use at an assay dependent dilution. Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/20000 is determined for one of the three peptides.
    WB: 1/1000. Predicted molecular weight: 48 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能
      Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
    • 組織特異性
      T-cells and endothelial cells.
    • 関連疾患
      Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
    • 配列類似性
      Contains 2 GATA-type zinc fingers.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
    • 製品の状態
      There are 2 isoforms produced by alternative splicing.
    • 別名
      • GATA 3 antibody
      • GATA binding factor 3 antibody
      • GATA binding protein 3 antibody
      • GATA-binding factor 3 antibody
      • Gata3 antibody
      • GATA3_HUMAN antibody
      • HDR antibody
      • HDRS antibody
      • MGC2346 antibody
      • MGC5199 antibody
      • MGC5445 antibody
      • Trans acting T cell specific transcription factor GATA 3 antibody
      • Trans-acting T-cell-specific transcription factor GATA-3 antibody
      see all

    Anti-GATA3 antibody 画像

    • All lanes : Anti-GATA3 antibody (ab82828) at 1/1000 dilution

      Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
      Lane 2 : induced culture of E.coli (one shot Top10 competent cells)

      Secondary
      Anti-Rabbit IgG HRP at 1/1000 dilution

      Predicted band size : 48 kDa
      Observed band size : 62 kDa (why is the actual band size different from the predicted?)

    プロトコール

    Anti-GATA3 antibody (ab82828) 使用論文

    ab82828 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. I have checked our catalog and found 4 anti-GATA3 antibodies that have been tested in mouse (ab32858, ab106625, ab82828 and ab110093), however none of these antibodies has been tested so far in frozen tissue section...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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