製品の概要

  • 製品名
    Anti-GATA3 antibody
    GATA3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GATA3
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    3 synthetic peptides (human) conjugated to KLH

  • ポジティブ･コントロール
    • Recombinant GATA3 protein expressed in induced culture of E.coli (one shot Top10 competent cells).

法規制情報

医薬用外毒物

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82828 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA
WB
  • 追加情報
    ELISA: Use at an assay dependent dilution. Antibody specificity was verified by direct ELISA against the 3 immunogen peptides. A minimum titer of 1/20000 is determined for one of the three peptides.
    WB: 1/1000. Predicted molecular weight: 48 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • ターゲット情報

    • 機能
      Transcriptional activator which binds to the enhancer of the T-cell receptor alpha and delta genes. Binds to the consensus sequence 5'-AGATAG-3'.
    • 組織特異性
      T-cells and endothelial cells.
    • 関連疾患
      Defects in GATA3 are the cause of hypoparathyroidism with sensorineural deafness and renal dysplasia (HDR) [MIM:146255]; also known as Barakat syndrome.
    • 配列類似性
      Contains 2 GATA-type zinc fingers.
    • 細胞内局在
      Nucleus.
    • Information by UniProt
    • 参照データベース
      • 製品の状態
        There are 2 isoforms produced by alternative splicing.
      • 別名
        • GATA 3 antibody
        • GATA binding factor 3 antibody
        • GATA binding protein 3 antibody
        • GATA-binding factor 3 antibody
        • Gata3 antibody
        • GATA3_HUMAN antibody
        • HDR antibody
        • HDRS antibody
        • MGC2346 antibody
        • MGC5199 antibody
        • MGC5445 antibody
        • Trans acting T cell specific transcription factor GATA 3 antibody
        • Trans-acting T-cell-specific transcription factor GATA-3 antibody
        see all

      画像

      • Western blot - GATA3 antibody (ab82828)
        Western blot - GATA3 antibody (ab82828)
        All lanes : Anti-GATA3 antibody (ab82828) at 1/1000 dilution

        Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
        Lane 2 : induced culture of E.coli (one shot Top10 competent cells)

        Secondary
        All lanes : Anti-Rabbit IgG HRP at 1/1000 dilution

        Predicted band size: 48 kDa
        Observed band size: 62 kDa (why is the actual band size different from the predicted?)



        Recombinant GATA3 protein is expressed using pBAD-DEST49 plasmid. Molecular weight of tag is 14kDa.

      プロトコール

      参考文献

      ab82828 has not yet been referenced specifically in any publications.

      ab82828 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

      レビューと Q&A

      レビューを送る 質問を送る

      1 Review or Q&A

      10
      Votes: Highest First

      I would like to use anti-GATA3 antibody for double immunoflouresecnce staining of mouse frozen sections.  Checking the web, I noticed that abcam has several antibodies for GATA3.  Which one would you recommend?   Thank you.

      Thank you for contacting us. I have checked our catalog and found 4 anti-GATA3 antibodies that have been tested in mouse (ab32858, ab106625, ab82828 and ab110093), however none of these antibodies has been tested so far in frozen tissue section...

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      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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