Anti-GATA1 抗体 [EP2819Y] (ab76121)


  • 製品名Anti-GATA1 antibody [EP2819Y]
    GATA1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP2819Y] to GATA1
  • アプリケーション適用あり: WB, Flow Cytmore details
    適用なし: ICC/IF,IHC-P or IP
  • 種交差性
    交差種: Human
  • 免疫原

    A synthetic peptide from the N terminus of human GATA1.

  • ポジティブ・コントロール
    • K562 cell lysate and permeabilized K562 cells
  • 特記事項

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    A trial size is available to purchase for this antibody.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.




Our Abpromise guarantee covers the use of ab76121 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/2000. Predicted molecular weight: 43 kDa.
Flow Cyt 1/50. ab172730-Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
  • 追加情報Is unsuitable for ICC/IF,IHC-P or IP.
  • ターゲット情報

    • 機能Transcriptional activator which probably serves as a general switch factor for erythroid development. It binds to DNA sites with the consensus sequence [AT]GATA[AG] within regulatory regions of globin genes and of other genes expressed in erythroid cells.
    • 組織特異性Erythrocytes.
    • 関連疾患Defects in GATA1 are the cause of X-linked dyserythropoietic anemia and thrombocytopenia (XDAT) [MIM:300367]. XDAT is a disorder characterized by erythrocytes with abnormal size and shape, and paucity of platelets in peripheral blood. The bone marrow contains abundant and abnormally small megakaryocytes.
      Defects in GATA1 are the cause of X-linked thrombocytopenia with beta-thalassemia (XLTT) [MIM:314050]; also knwon as thrombocytopenia, platelet dysfunction, hemolysis, and imbalanced globin synthesis. XLTT consists of an unusual form of thrombocytopenia with beta-thalassemia. Patients have splenomegaly and petechiae, moderate thrombocytopenia, prolonged bleeding time due to platelet dysfunction, reticulocytosis and unbalanced hemoglobin chain synthesis resembling that of beta-thalassemia minor.
      Defects in GATA1 are the cause of anemia without thrombocytopenia X-linked (XLAWT) [MIM:300835]. XLAWT is a form of anemia characterized by abnormal morphology of erythrocytes and granulocytes in peripheral blood, bone marrow dysplasia with hypocellularity of erythroid and granulocytic lineages, and normal or increased number of megakaryocytes. Neutropenia of a variable degree is present in affected individuals.
    • 配列類似性Contains 2 GATA-type zinc fingers.
    • ドメインThe two fingers are functionally distinct and cooperate to achieve specific, stable DNA binding. The first finger is necessary only for full specificity and stability of binding, whereas the second one is required for binding.
    • 翻訳後修飾Highly phosphorylated on serine residues. Phosphorylation on Ser-310 is enhanced on erythroid differentiation. Phosphorylation on Ser-142 promotes sumoylation on Lys-137.
      Sumoylation on Lys-137 is enhanced by phosphorylation on Ser-142 and by interaction with PIAS4. Sumoylation by SUMO1 has no effect on transcriptional activity.
    • 細胞内局在Nucleus.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Anemia, X-linked, without thrombocytopenia, included antibody
      • ERYF 1 antibody
      • Eryf1 antibody
      • Erythroid transcription factor antibody
      • Erythrold transcription factor 1 antibody
      • GATA 1 antibody
      • GATA binding factor 1 antibody
      • GATA binding protein 1 (globin transcription factor 1) antibody
      • GATA binding protein 1 antibody
      • GATA-1 antibody
      • GATA-binding factor 1 antibody
      • GATA1 antibody
      • GATA1_HUMAN antibody
      • GF 1 antibody
      • GF-1 antibody
      • GF1 antibody
      • Globin transcription factor 1 antibody
      • NF E1 antibody
      • NF E1 DNA binding protein antibody
      • NF-E1 DNA-binding protein antibody
      • NFE 1 antibody
      • NFE1 antibody
      • Nuclear factor erythroid 1 antibody
      • Transcription factor GATA1 antibody
      • XLANP antibody
      • XLTDA antibody
      • XLTT antibody
      see all

    Anti-GATA1 antibody [EP2819Y] 画像

    • Anti-GATA1 antibody [EP2819Y] (ab76121) at 1/2000 dilution + K562 cell lysate at 10 µg

      HRP labelled goat anti-rabbit at 1/1000 dilution

      Predicted band size : 43 kDa
      Observed band size : 43 kDa
    • Flow cytometric analysis of permeabilized K562 cells using ab76121 at a 1/50 dilution (red) or a rabbit IgG (negative) (green).

    Anti-GATA1 antibody [EP2819Y] (ab76121) 使用論文

    ab76121 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for contacting us. The antibody was testing using the protocol below. The rabbit monoclonal isotyope control is ab125938.

    1. Solutions and Reagents

    1.1. 1X PBS

    1.2. Blocking buffer: 0.5% BSA in 1X PBS


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