Anti-GAS2 抗体 [EPR1742(2)] (ab109762)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1742(2)] to GAS2
- Suitable for: WB, IHC-P
- Reacts with: Human
製品の概要
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製品名
Anti-GAS2 antibody [EPR1742(2)] -
製品の詳細
Rabbit monoclonal [EPR1742(2)] to GAS2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details
適用なし: Flow Cyt or ICC/IF -
種交差性
交差種: Human
交差が予測される動物種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- HepG2, fetal liver, fetal kidney, and Jurkat lysates, Human stomach tissue
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR1742(2) -
アイソタイプ
IgG -
研究分野
関連製品
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab109762の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa). |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
May play a role in apoptosis by acting as a cell death substrate for caspases. Is cleaved during apoptosis and the cleaved form induces dramatic rearrangements of the actin cytoskeleton and potent changes in the shape of the affected cells. May be involved in the membrane ruffling process. -
組織特異性
Ubiquitously expressed with highest levels in liver, lung, and kidney. Not found in spleen. -
配列類似性
Belongs to the GAS2 family.
Contains 1 CH (calponin-homology) domain.
Contains 1 GAR domain. -
発生段階
Specifically expressed at growth arrest. -
翻訳後修飾
Cleaved, during apoptosis, on a specific aspartic residue by caspases.
Phosphorylated on serine residues during the G0-G1 transition phase. -
細胞内局在
Cytoplasm > cytoskeleton. Membrane. Component of the microfilament system. Colocalizes with actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Mainly membrane-associated. When hyperphosphorylated, accumulates at membrane ruffles. - Information by UniProt
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参照データベース
- Entrez Gene: 2620 Human
- Entrez Gene: 14453 Mouse
- Omim: 602835 Human
- SwissProt: O43903 Human
- SwissProt: P11862 Mouse
- Unigene: 632151 Human
- Unigene: 207360 Mouse
- Unigene: 386823 Mouse
see all -
別名
- GAS 2 antibody
- GAS-2 antibody
- Gas2 antibody
see all
画像
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All lanes : Anti-GAS2 antibody [EPR1742(2)] (ab109762) at 1/1000 dilution
Lane 1 : HepG2 lysate
Lane 2 : Human fetal liver lysate
Lane 3 : Human fetal kidney lysate
Lane 4 : Jurkat lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 35 kDa
Observed band size: 35 kDa -
Immunohistochemical analysis of paraffin-embedded Human stomach tissue using ab109762 at a dilution of 1/50.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab109762 は 4 報の論文で使用されています。
- Ma W et al. Growth arrest-specific protein 2 (GAS2) interacts with CXCR4 to promote T-cell leukemogenesis partially via c-MYC. Mol Oncol 16:3720-3734 (2022). PubMed: 36054080
- Sun N et al. DCX and CRABP2 are candidate genes for differential diagnosis between pre-chemotherapy embryonic and alveolar rhabdomyosarcoma in pediatric patients. Pediatr Investig 5:106-111 (2021). PubMed: 34179706
- Shukla S et al. Aberrant Activation of a Gastrointestinal Transcriptional Circuit in Prostate Cancer Mediates Castration Resistance. Cancer Cell 32:792-806.e7 (2017). PubMed: 29153843
- Sun L et al. GAS2-Calpain2 axis contributes to the growth of leukemic cells. Acta Biochim Biophys Sin (Shanghai) 47:795-804 (2015). PubMed: 26358320