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RabMAb

Anti-GAS2 抗体 [EPR1742(2)] (ab109762)

製品の概要

  • 製品名
    Anti-GAS2 antibody [EPR1742(2)]
    GAS2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR1742(2)] to GAS2
  • アプリケーション
    適用あり: WB, IP, IHC-Pmore details
    適用なし: Flow Cyt or ICC
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to residues on the N-terminus in Human GAS2.

  • ポジティブ・コントロール
    • HepG2, fetal liver, fetal kidney, and Jurkat lysates, Human stomach tissue
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab109762 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).
IP 1/10 - 1/100.
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for Flow Cyt or ICC.
  • ターゲット情報

    • 機能
      May play a role in apoptosis by acting as a cell death substrate for caspases. Is cleaved during apoptosis and the cleaved form induces dramatic rearrangements of the actin cytoskeleton and potent changes in the shape of the affected cells. May be involved in the membrane ruffling process.
    • 組織特異性
      Ubiquitously expressed with highest levels in liver, lung, and kidney. Not found in spleen.
    • 配列類似性
      Belongs to the GAS2 family.
      Contains 1 CH (calponin-homology) domain.
      Contains 1 GAR domain.
    • 発生段階
      Specifically expressed at growth arrest.
    • 翻訳後修飾
      Cleaved, during apoptosis, on a specific aspartic residue by caspases.
      Phosphorylated on serine residues during the G0-G1 transition phase.
    • 細胞内局在
      Cytoplasm > cytoskeleton. Membrane. Component of the microfilament system. Colocalizes with actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Mainly membrane-associated. When hyperphosphorylated, accumulates at membrane ruffles.
    • Information by UniProt
    • 参照データベース
    • 別名
      • GAS 2 antibody
      • GAS-2 antibody
      • Gas2 antibody
      • GAS2_HUMAN antibody
      • Growth arrest specific 2 antibody
      • Growth arrest specific protein 2 antibody
      • Growth arrest-specific protein 2 antibody
      • MGC32610 antibody
      see all

    Anti-GAS2 antibody [EPR1742(2)] 画像

    • All lanes : Anti-GAS2 antibody [EPR1742(2)] (ab109762) at 1/1000 dilution

      Lane 1 : HepG2 lysate
      Lane 2 : Human fetal liver lysate
      Lane 3 : Human fetal kidney lysate
      Lane 4 : Jurkat lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 35 kDa
      Observed band size : 35 kDa
    • Immunohistochemical analysis of paraffin-embedded Human stomach tissue using ab109762 at a dilution of 1/50.

    Anti-GAS2 antibody [EPR1742(2)] (ab109762) 使用論文

    This product has been referenced in:
    • Sun L  et al. GAS2-Calpain2 axis contributes to the growth of leukemic cells. Acta Biochim Biophys Sin (Shanghai) 47:795-804 (2015). Read more (PubMed: 26358320) »

    See 1 Publication for this product

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    There are currently no Abreviews or Questions for ab109762.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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