製品の概要

  • 製品名Anti-GAS2 antibody
    GAS2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GAS2
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rabbit, Horse, Cow, Macaque Monkey, Gorilla
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human GAS2.

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human and Mouse Kidney tissue lysate as well as both HEK293 and HepG2 whole cell lysate.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーpH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab106505 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 35 kDa (predicted molecular weight: 35 kDa).

ターゲット情報

  • 機能May play a role in apoptosis by acting as a cell death substrate for caspases. Is cleaved during apoptosis and the cleaved form induces dramatic rearrangements of the actin cytoskeleton and potent changes in the shape of the affected cells. May be involved in the membrane ruffling process.
  • 組織特異性Ubiquitously expressed with highest levels in liver, lung, and kidney. Not found in spleen.
  • 配列類似性Belongs to the GAS2 family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 GAR domain.
  • 発生段階Specifically expressed at growth arrest.
  • 翻訳後修飾Cleaved, during apoptosis, on a specific aspartic residue by caspases.
    Phosphorylated on serine residues during the G0-G1 transition phase.
  • 細胞内局在Cytoplasm > cytoskeleton. Membrane. Component of the microfilament system. Colocalizes with actin fibers at the cell border and along the stress fibers in growth-arrested fibroblasts. Mainly membrane-associated. When hyperphosphorylated, accumulates at membrane ruffles.
  • Information by UniProt
  • 参照データベース
  • 別名
    • GAS 2 antibody
    • GAS-2 antibody
    • Gas2 antibody
    • GAS2_HUMAN antibody
    • Growth arrest specific 2 antibody
    • Growth arrest specific protein 2 antibody
    • Growth arrest-specific protein 2 antibody
    • MGC32610 antibody
    see all

Anti-GAS2 antibody 画像

  • All lanes : Anti-GAS2 antibody (ab106505) at 1 µg/ml

    Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 2 : Human kidney tissue lysate - total protein (ab30203)
    Lane 3 : Kidney (Mouse) Tissue Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 35 kDa
    Observed band size : 35 kDa
    Additional bands at : 73 kDa,89 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 2 minutes

Anti-GAS2 antibody (ab106505) 使用論文

ab106505 has not yet been referenced specifically in any publications.

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