製品の概要

  • 製品名Anti-GAS 6 antibody
    GAS 6 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GAS 6
  • アプリケーション適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 650 to the C-terminus of Human GAS 6.

    (Peptide available as ab97363.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Human Heart Tissue Lysate.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab85388 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 79 kDa).

ターゲット情報

  • 機能Ligand for tyrosine-protein kinase receptors AXL, TYRO3 and MER whose signaling is implicated in cell growth and survival, cell adhesion and cell migration. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses.
  • 組織特異性Plasma. Isoform 1 and isoform 2 are widely expressed. Isoform 1 is the predominant form in spleen.
  • 配列類似性Contains 4 EGF-like domains.
    Contains 1 Gla (gamma-carboxy-glutamate) domain.
    Contains 2 laminin G-like domains.
  • 翻訳後修飾Isoform 1 is proteolytically processed after secretion to yield a N-terminal 36 kDa protein and a C-terminal 50 kDa protein including the laminin G-like domains which activates AXL.
    Gamma-carboxyglutamate residues are formed by vitamin K dependent carboxylation. These residues are essential for the binding of calcium.
  • 細胞内局在Secreted.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AXL receptor tyrosine kinase ligand antibody
    • AXL stimulatory factor antibody
    • AXLLG antibody
    • AXS F antibody
    • AXSF antibody
    • DKFZp666G247 antibody
    • FLJ34709 antibody
    • Gas 6 antibody
    • GAS-6 antibody
    • Gas6 antibody
    • GAS6_HUMAN antibody
    • Growth arrest specific 6 antibody
    • Growth arrest-specific protein 6 antibody
    • Growth-arrest-specific protein 6 antibody
    • Growth-arrest-specific protein 6 precursor antibody
    see all

Anti-GAS 6 antibody 画像

  • Anti-GAS 6 antibody (ab85388) at 1 µg/ml + Human heart tissue lysate - total protein (ab29431) at 10 µg

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 79 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 37 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 minutes

Anti-GAS 6 antibody (ab85388) 使用論文

ab85388 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (macrophage)
Specification macrophage
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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投稿 Dec 22 2014

Application Western blot
Loading amount 1e+006 cells
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (primary human macrophages)
Specification primary human macrophages
Treatment MDP
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
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Verified customer

投稿 Feb 12 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"