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RabMAb

Anti-GARS 抗体 [EPR7157] (ab125008)

製品の概要

  • 製品名
    Anti-GARS antibody [EPR7157]
    GARS 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR7157] to GARS
  • アプリケーション
    適用あり: WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide, corresponding to residues near the C-terminus of Human GARS.

  • ポジティブ・コントロール
    • Jurkat, HeLa, U87-MG, 293T, C6, RAW 264.7, and Neuro-2a cell lysates; Human colon tissue.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • バッファー
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 49% PBS, 50% Glycerol, 0.05% BSA
  • 精製度
    Tissue culture supernatant
  • ポリ/モノ
    モノクローナル
  • クローン名
    EPR7157
  • アイソタイプ
    IgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab125008 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 75 kDa (predicted molecular weight: 83 kDa).
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC or IP.
  • ターゲット情報

    Anti-GARS antibody [EPR7157] 画像

    • All lanes : Anti-GARS antibody [EPR7157] (ab125008) at 1/1000 dilution

      Lane 1 : Jurkat cell lysate
      Lane 2 : Hela cell lysate
      Lane 3 : U87-MG cell lysate
      Lane 4 : 293T cell lysate
      Lane 5 : C6 cell lysate
      Lane 6 : RAW 264.7 cell lysate
      Lane 7 : Neuro-2a cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 83 kDa
      Observed band size : 75 kDa (why is the actual band size different from the predicted?)
    • ab125008 at 1/50 staining GARS in paraffin embedded Human colon tissue by immunohistochemistry.

    Anti-GARS antibody [EPR7157] (ab125008) 使用論文

    ab125008 has not yet been referenced specifically in any publications.

    Product Wall

    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (HeLa)
    Specification
    HeLa
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Fixative
    Paraformaldehyde
    Username

    Dr. Kirk Mcmanus

    Verified customer

    投稿 Apr 04 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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