製品の概要

  • 製品名
  • 製品の詳細
    Mouse polyclonal to GARS
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Full length human GARS protein (ENSP00000373918; or amino acids 55-739 of Swissprot id P41250).

  • ポジティブ・コントロール
    • Human placenta tissue lysate; GARS transfected 293T cell lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • 精製度
    Protein A purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab89522 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 75 kDa.

ターゲット情報

  • 関連性
    GARS synthetase catalyzes the ligation of glycine with glycyl-tRNA using ATP and its mutation induces Charcot-Marie-Tooth (CMT) disease.
  • 細胞内局在
    Cytoplasm. Mitochondrion.
  • 参照データベース
  • 別名
    • AP 4 A synthetase antibody
    • Charcot Marie Tooth neuropathy 2D antibody
    • Charcot Marie Tooth neuropathy neuronal type D antibody
    • CMT2D antibody
    • Diadenosine tetraphosphate synthetase antibody
    • DSMAV antibody
    • EC 6.1.1.14 antibody
    • Glycine tRNA ligase antibody
    • Glycyl tRNA synthetase antibody
    • GlyRS antibody
    • HMN5 antibody
    • SMAD1 antibody
    see all

画像

  • Anti-GARS antibody (ab89522) at 1 µg/ml + human placenta tissue lysate at 50 µg

    Predicted band size : 75 kDa
    Observed band size : ~75 kDa (why is the actual band size different from the predicted?)
  • All lanes : Anti-GARS antibody (ab89522) at 1 µg/ml

    Lane 1 : GARS transfected 293T cell lysate
    Lane 2 : Non transfected lysate

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 75 kDa
    Observed band size : 75 kDa

プロトコール

参考文献

This product has been referenced in:
  • Nafisinia M  et al. Compound heterozygous mutations in glycyl-tRNA synthetase (GARS) cause mitochondrial respiratory chain dysfunction. PLoS One 12:e0178125 (2017). WB ; Human . Read more (PubMed: 28594869) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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