製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to GARS
  • 特異性
    This antibody reacts specifically with 79kDa GARS protein.
  • アプリケーション
    適用あり: ICC/IF, IP, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Recombinant full length protein (Human)

  • ポジティブ・コントロール
    • HEK293 cell lysate.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: Whole serum
  • 精製度
    Whole antiserum
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab42905 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/3000 - 1/10000.
IP 1/3000 - 1/10000.
WB 1/5000. Predicted molecular weight: 83 kDa.

ターゲット情報

  • 関連性
    GARS synthetase catalyzes the ligation of glycine with glycyl-tRNA using ATP and its mutation induces Charcot-Marie-Tooth (CMT) disease.
  • 細胞内局在
    Cytoplasm. Mitochondrion.
  • 参照データベース
  • 別名
    • AP 4 A synthetase antibody
    • Charcot Marie Tooth neuropathy 2D antibody
    • Charcot Marie Tooth neuropathy neuronal type D antibody
    • CMT2D antibody
    • Diadenosine tetraphosphate synthetase antibody
    • DSMAV antibody
    • EC 6.1.1.14 antibody
    • Glycine tRNA ligase antibody
    • Glycyl tRNA synthetase antibody
    • GlyRS antibody
    • HMN5 antibody
    • SMAD1 antibody
    see all

Anti-GARS antibody 画像

  • Anti-GARS antibody (ab42905) at 1/5000 dilution + whole cell lysate prepared from HEK293 cells at 50 µg/ml

    Secondary
    Goat anti-rabbit IgG (H+L) at 1/10000 dilution

    Predicted band size : 83 kDa
    Observed band size : 75 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab42905 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab42905, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab42905 staining GARS in murine sciatic nerve tissue by Immunohistochemistry (PFA perfusion fixed frozen sections).

    Tissue was fixed with paraformaldehyde and blocked with 5% goat serum in PBS with 0.5% Triton X-100 for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in diluent) overnight at 4°C. An AlexaFluor®555-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.

Anti-GARS antibody (ab42905) 使用論文

This product has been referenced in:
  • Niehues S  et al. Impaired protein translation in Drosophila models for Charcot-Marie-Tooth neuropathy caused by mutant tRNA synthetases. Nat Commun 6:7520 (2015). Read more (PubMed: 26138142) »
  • Stum M  et al. An assessment of mechanisms underlying peripheral axonal degeneration caused by aminoacyl-tRNA synthetase mutations. Mol Cell Neurosci 46:432-43 (2011). Read more (PubMed: 21115117) »

See all 4 Publications for this product

Product Wall

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Fixative
Paraformaldehyde
Username

Dr. Kirk Mcmanus

Verified customer

投稿 Aug 21 2014

Application
Western blot
Sample
Rat Cell lysate - whole cell (C6 Rat glioma cells)
Loading amount
40 µg
Specification
C6 Rat glioma cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Sep 12 2012


Thank you for your inquiry. We are happy to provide the discount code to the customer for testing ab42905 in rat. As you know, this code will give you: 1 free primary antibody before the expiration date. To redeem this offer, the customer must sub...

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (neuron)
Total protein in input
15 µg
Specification
neuron
Immuno-precipitation step
Protein G
Username

Abcam user community

Verified customer

投稿 May 22 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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