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Full length native protein from human erythrocytes.
Our Abpromise guarantee covers the use of ab9483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Detects a band of approximately 37 kDa (predicted molecular weight: 35.8 kDa).|
|ICC/IF||Use a concentration of 1 µg/ml.|
ab9483 staining GAPDH in HeLa by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehydeand. Samples were incubated with primary antibody (1/200) for 30 minutes. An undiluted Alexa Fluor® 568-conjugated Donkey anti-goat polyclonal was used as the secondary antibody.