製品の概要

  • 製品名Anti-GAPDH antibody (HRP)
    GAPDH 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to GAPDH (HRP)
  • 標識HRP
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Goat, Chicken, Hamster, Cow, Human, Pig, Fish, Escherichia coli
  • 免疫原

    Full length native GAPDH protein purified from Rabbit muscle.

  • ポジティブ・コントロール
    • Breast tissue.

法規制情報

アプリケーション

Our Abpromise guarantee covers the use of ab85760 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution. Predicted molecular weight: 36 kDa.
IHC-P Use a concentration of 2.5 µg/ml.

ターゲット情報

  • 機能Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • パスウェイCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • 配列類似性Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • 翻訳後修飾S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • 細胞内局在Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Information by UniProt
  • 参照データベース
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Anti-GAPDH antibody (HRP) 画像

  • ab85760 at 2.5µg/ml staining GAPDH in breast tissue by Immunohistochemistry (Formalin-fixed paraffin-embedded section).

Anti-GAPDH antibody (HRP) (ab85760) 使用論文

This product has been referenced in:
  • Sachdeva G  et al. In vivo co-localization of enzymes on RNA scaffolds increases metabolic production in a geometrically dependent manner. Nucleic Acids Res 42:9493-503 (2014). WB . Read more (PubMed: 25034694) »
  • Langiu M  et al. Human serum albumin-based nanoparticle-mediated in vitro gene delivery. PLoS One 9:e107603 (2014). Dot blot ; Human . Read more (PubMed: 25229502) »

See all 2 Publications for this product

Product Wall

I would be very happy to provide an alternative product against GAPDH in E.coli. We do carry one anti-GAPDH which is tested and guaranteed in E.coli. This product is ab85760. This product is conjugated to HRP so you will not need to use a secondary ant...

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When choosing a loading control it is best practice to choose housekeeping genes which will be present in your sample type. For example, in whole cell preparations beta Actin or GAPDH will be appropriate while ...

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