製品の概要

  • 製品名
    Anti-GAPDH antibody [GA1R]
    GAPDH 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [GA1R] to GAPDH
  • 由来種
    Mouse
  • 特異性
    ab125247 recognizes native and denatured forms of GAPDH.
  • アプリケーション
    適用あり: WB, Dot blot, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Chicken, Hamster, Human, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Other
    交差が予測される動物種: a wide range of other species
  • 免疫原

    Recombinant GAPDH.

  • ポジティブ･コントロール
    • Human, Mouse, Rat, Rabbit, Chicken and Hamster tissue lysates; BL-21 bacteria, Sf9 insect and Saccharomyces cerevisiae cell lysates
  • 特記事項

    Store at 4°C (add 0.05% NaN3) for several days to weeks.

    This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab125247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa). with ECL.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

ターゲット情報

画像

  • Western blot - Anti-GAPDH antibody [GA1R] (ab125247)
    Western blot - Anti-GAPDH antibody [GA1R] (ab125247)
    All lanes : Anti-GAPDH antibody [GA1R] (ab125247) at 1/2000 dilution

    Lane 1 : Human tissue lysate
    Lane 2 : Mouse tissue lysate
    Lane 3 : Rat tissue lysate
    Lane 4 : Rabbit tissue lysate
    Lane 5 : Chicken tissue lysate
    Lane 6 : Hamster tissue lysate

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 36 kDa

  • Western blot - Anti-GAPDH antibody [GA1R] (ab125247)
    Western blot - Anti-GAPDH antibody [GA1R] (ab125247)
    All lanes : Anti-GAPDH antibody [GA1R] (ab125247) at 1/2000 dilution

    Lane 1 : BL21 bacterial lysate
    Lane 2 : Sf9 insect lysate
    Lane 3 : Saccharomyces cerevisiae lysate

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 36 kDa

プロトコール

参考文献

This product has been referenced in:
  • Ye Y  et al. microRNA-605 promotes cell proliferation, migration and invasion in non-small cell lung cancer by directly targeting LATS2. Exp Ther Med 14:867-873 (2017). Read more (PubMed: 28673012) »
  • Sorel O  et al. Macavirus latency-associated protein evades immune detection through regulation of protein synthesis in cis depending upon its glycin/glutamate-rich domain. PLoS Pathog 13:e1006691 (2017). WB . Read more (PubMed: 29059246) »

See all 19 Publications for this product

ab125247 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

レビューと Q&A

レビューを送る 質問を送る

13 reviews or Q&A

10
Votes: Highest First

Western blot abreview for Anti-GAPDH antibody [GA1R]

 Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (THP-1, A549, HEK293, CEMx-174)
Gel Running Conditions
Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Loading amount
5 µg
Treatment
Cells were mixed in Ripa buffer and denaturated in Laemmli buffer 5min à 95°C
Specification
THP-1, A549, HEK293, CEMx-174
Blocking step
Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Read More
abreview image
Username

Dr. Francois Daubeuf

Verified customer

投稿 Oct 20 2015

Western blot abreview for Anti-GAPDH antibody [GA1R] - Loading Control

 Good
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
3.75e+006 cells
Gel Running Conditions
Reduced Denaturing (NUPAGE precasted Invitrogen 10% SDS-PAGE)
Sample
Schizosaccharomyces pombe Cell lysate - whole cell (whole cell extract)
Specification
whole cell extract
Blocking step
0.5% BSA + 5% non fat milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Read More
abreview image
Username

Abcam user community

Verified customer

投稿 Jul 17 2013

Hi

Thank you very much for your reply.

I have tried the replacement antibody and we got it to work in the first shot using a slightly modified protocol from the one that didn’t work initially: 1:500 dilution and overnight incuba...

Read More

Thank you for that feedback. I am pleased to hear that the replacement antibody has proved successful for you.

I do not completely understand why at a dilution of 1/500 you would only be able to perform two western blots. There should be enoug...

Read More

Thank you for sending a replacement antibody. We’ll be running some tests once we have received a more detailed reference protocol from you and can hopefully get it working.

Yes, the valproic acid has been used in an assay as a reference...

Read More

I am very sorry for the delay in getting back to you in regards to the Western blotting protocol used with the Anti-GAPDH antibody [GA1R] ab125247.

I can now confirm that the following conditions were used to obtain the image presented on the ...

Read More

hello, we would need to use a gdph antibody for fly brain. do you know if that one will work.

Thank you very much for contacting Abcam.

To our knowledge, ab125247 has not been tested in Fly/Drosophila tissue. Therefore, I can offer you our AbTrial program where you can now use our products in an untested application or species without ...

Read More

Dear xxxxx
Thank you for sending a replacement antibody. We’ll be running some tests once we have received a more detailed reference protocol from you and can hopefully get it working.

Yes, the valproic acid has been used in an as...

Read More

Thank you for getting back to me.

I think the protocol you have been using should be suitable. I would however make sure that the samples are sufficiently lysed, using RIPA buffer and loading onto the gel using Laminae buffer with reducing ag...

Read More

In reply to your questitions:

Yes the RIPA buffer gave a color change of the Bradford reagent. But we do not wish to use the RIPA buffer as it contains detergent, which we want to avoid because it might extract some of our membrane protein. ...

Read More

Thank you for your reply.

I would be concerned that you are getting a colour change in your Bradford reagent without any sample present. This would suggest that there is a contamination in your buffers. I would make new sterile ones to make s...

Read More

Order date: 17th of july

I’m overexpressing in sf9 cells yes and detecting the poorly membrane protein by the FLAG tag. This protein is approximately 56 kDa but ruuning on the gels close to 40 kDa as it is a membrane protein.

Read More

Thank you for your reply.

I think it is very important to check how much protein is being loaded to keep it within a manageable range, loading too much can lead to aggregation of proteins and high background whilst loading too little can obvi...

Read More

Hi
I’ll have to get back to you with the order number as our technician has gone for the day, but is probably ordered in june or july. The first experiments have been done in late july.

The antibody for the membrane protein is an ...

Read More

Thank you for confirming that information.

It would be helpful to have the order number if possible. I have found one order of the 17th of July delivered to the University of Copenhagen, Faculty of Health Sciences. Is this your order?
Read More

Product code: 125247
Lot number: GR80148-4

Inquiry: The problem seems to be unspecific binding of some sort/ no specific binding at all. We ant to detect GAPDH in sf9 cells.The exact protein concentration is unknown, but we are able to ...

Read More

Thank you for sharing this information.

I am currently consulting with the source of this antibody to try to understand why the results have not been as expected with this antibody.

In order to investigate the situation further, cou...

Read More

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録