製品の概要

  • 製品名Anti-GAPDH antibody [GA1R]
    GAPDH 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [GA1R] to GAPDH
  • 特異性ab125247 recognizes native and denatured forms of GAPDH.
  • アプリケーション適用あり: WB, Dot blot, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Chicken, Hamster, Human, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Other
    交差が予測される動物種: a wide range of other species
  • 免疫原

    Recombinant GAPDH.

  • ポジティブ・コントロール
    • Human, Mouse, Rat, Rabbit, Chicken and Hamster tissue lysates; BL-21 bacteria, Sf9 insect and Saccharomyces cerevisiae cell lysates
  • 特記事項

    Store at 4°C (add 0.05% NaN3) for several days to weeks.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab125247 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000 - 1/10000. Detects a band of approximately 37 kDa (predicted molecular weight: 36 kDa). with ECL.
Dot blot Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF Use at an assay dependent concentration.

ターゲット情報

Anti-GAPDH antibody [GA1R] 画像

  • All lanes : Anti-GAPDH antibody [GA1R] (ab125247) at 1/2000 dilution

    Lane 1 : Human tissue lysate
    Lane 2 : Mouse tissue lysate
    Lane 3 : Rat tissue lysate
    Lane 4 : Rabbit tissue lysate
    Lane 5 : Chicken tissue lysate
    Lane 6 : Hamster tissue lysate

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique

    Predicted band size : 36 kDa
  • All lanes : Anti-GAPDH antibody [GA1R] (ab125247) at 1/2000 dilution

    Lane 1 : BL21 bacterial lysate
    Lane 2 : Sf9 insect lysate
    Lane 3 : Saccharomyces cerevisiae lysate

    Lysates/proteins at 5 µg per lane.

    Developed using the ECL technique

    Predicted band size : 36 kDa

Anti-GAPDH antibody [GA1R] (ab125247) 使用論文

This product has been referenced in:
  • Ren YQ  et al. MiR-27a modulates radiosensitivity of triple-negative breast cancer (TNBC) cells by targeting CDC27. Med Sci Monit 21:1297-303 (2015). WB ; Human . Read more (PubMed: 25943633) »
  • Liu W  et al. miR-221 and miR-222 Simultaneously Target RECK and Regulate Growth and Invasion of Gastric Cancer Cells. Med Sci Monit 21:2718-25 (2015). WB ; Human . Read more (PubMed: 26364844) »

See all 6 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (THP-1, A549, HEK293, CEMx-174)
Gel Running Conditions Reduced Denaturing (Precast Gel 4-12%, Criterion TGX (Biorad))
Loading amount 5 µg
Treatment Cells were mixed in Ripa buffer and denaturated in Laemmli buffer 5min à 95°C
Specification THP-1, A549, HEK293, CEMx-174
Blocking step Milk as blocking agent for 1 minute(s) · Concentration: 0.5% · Temperature: 20°C
Username

Dr. Francois Daubeuf

Verified customer

投稿 Oct 20 2015

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 3.75e+006 cells
Gel Running Conditions Reduced Denaturing (NUPAGE precasted Invitrogen 10% SDS-PAGE)
Sample Schizosaccharomyces pombe Cell lysate - whole cell (whole cell extract)
Specification whole cell extract
Blocking step 0.5% BSA + 5% non fat milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Jul 17 2013

Thank you for that feedback. I am pleased to hear that the replacement antibody has proved successful for you.

I do not completely understand why at a dilution of 1/500 you would only be able to perform two western blots. There should be enoug...

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I am very sorry for the delay in getting back to you in regards to the Western blotting protocol used with the Anti-GAPDH antibody [GA1R] ab125247.

I can now confirm that the following conditions were used to obtain the image presented on the ...

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Thank you very much for contacting Abcam.

To our knowledge, ab125247 has not been tested in Fly/Drosophila tissue. Therefore, I can offer you our AbTrial program where you can now use our products in an untested application or species without ...

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Thank you for your reply.

I would be concerned that you are getting a colour change in your Bradford reagent without any sample present. This would suggest that there is a contamination in your buffers. I would make new sterile ones to make s...

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Thank you for your reply.

I think it is very important to check how much protein is being loaded to keep it within a manageable range, loading too much can lead to aggregation of proteins and high background whilst loading too little can obvi...

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Thank you for confirming that information.

It would be helpful to have the order number if possible. I have found one order of the 17th of July delivered to the University of Copenhagen, Faculty of Health Sciences. Is this your order?
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Thank you for sharing this information.

I am currently consulting with the source of this antibody to try to understand why the results have not been as expected with this antibody.

In order to investigate the situation further, cou...

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Thank you for contacting us. I am very glad to hear that you have taken advantage of our 100% Abreview program.

I have found the review which you have submitted and wish to thank you for this. In order to take advantage of the review code,...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"