Anti-GAPDH 抗体 - Carboxyterminal end (ab62489)

製品の概要

  • 製品名Anti-GAPDH antibody - Carboxyterminal end
    GAPDH 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to GAPDH - Carboxyterminal end
  • 特異性This antibody reacts with GAPDH
  • アプリケーション適用あり: ICC/IF, ICC, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A 16 amino acid synthetic peptide from near the carboxy-terminus of human GAPDH

  • ポジティブ・コントロール
    • Hela cell lysates

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 20 µg/ml.
ICC Use a concentration of 10 µg/ml.
WB Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa).

ターゲット情報

  • 機能Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • パスウェイCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • 配列類似性Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • 翻訳後修飾S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • 細胞内局在Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Aging associated gene 9 protein antibody
    • Epididymis secretory sperm binding protein Li 162eP antibody
    • G3P_HUMAN antibody
    • G3PD antibody
    • G3PDH antibody
    • GAPD antibody
    • GAPDH antibody
    • Glyceraldehyde 3 phosphate dehydrogenase antibody
    • Glyceraldehyde-3-phosphate dehydrogenase antibody
    • HEL S 162eP antibody
    • Peptidyl-cysteine S-nitrosylase GAPDH antibody
    see all

Anti-GAPDH antibody - Carboxyterminal end 画像

  • Lane 1 : Anti-GAPDH antibody - Carboxyterminal end (ab62489) at 0.5 µg/ml
    Lane 2 : Anti-GAPDH antibody - Carboxyterminal end (ab62489) at 1 µg/ml
    Lane 3 : Anti-GAPDH antibody - Carboxyterminal end (ab62489) at 2 µg/ml

    Lane 1 : Hela cell lysate
    Lane 2 : Hela cell lysate
    Lane 3 : Hela cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 36 kDa
    Observed band size : 39 kDa (why is the actual band size different from the predicted?)
  • ab62489 at 10µg/ml staining GAPDH in human Hela cells by Imunocytochemistry. 

  • Immunofluorescence of GAPDH in HeLa cells using ab62489 at 20 ug/ml.

Anti-GAPDH antibody - Carboxyterminal end (ab62489) 使用論文

ab62489 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"