The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 20 µg/ml.
Use a concentration of 10 µg/ml.
Use a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 36 kDa).
Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus. ISGylated.
Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
Epididymis secretory sperm binding protein Li 162eP antibody
Glyceraldehyde 3 phosphate dehydrogenase antibody
Glyceraldehyde-3-phosphate dehydrogenase antibody
HEL S 162eP antibody
Peptidyl-cysteine S-nitrosylase GAPDH antibody
Anti-GAPDH antibody - Carboxyterminal end 画像
Western blot - GAPDH antibody - Carboxyterminal end (ab62489)
Lane 1 : Anti-GAPDH antibody - Carboxyterminal end (ab62489) at 0.5 µg/ml Lane 2 : Anti-GAPDH antibody - Carboxyterminal end (ab62489) at 1 µg/ml Lane 3 : Anti-GAPDH antibody - Carboxyterminal end (ab62489) at 2 µg/ml
Lane 1 : Hela cell lysate Lane 2 : Hela cell lysate Lane 3 : Hela cell lysate