製品の概要
- 製品名Anti-GAPDH antibody
GAPDH 一次抗体 製品一覧 - 製品の詳細Chicken polyclonal to GAPDH
- アプリケーション適用あり: WB, ELISA, ICC/IFmore details
- 種交差性交差種: Mouse, Rat, Human
- 免疫原
A 16 amino acid synthetic peptide from near the carboxy-terminus of Human GAPDH (Swiss-Prot P04406).
- ポジティブ･コントロール
- HeLa Cell Lysate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa
製品の特性
- 製品の状態Liquid
- 保存方法Shipped at 4°C. Store at +4°C.
- バッファーPreservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... - 精製度Immunogen affinity purified
- ポリ/モノポリクローナル
- アイソタイプIgY
- 研究分野
関連製品
- Compatible Secondaries
- Related Products
アプリケーション
Our Abpromise guarantee covers the use of ab83956 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 36 kDa. | |
| ELISA | Use at an assay dependent concentration. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
ターゲット情報
- 機能Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
- パスウェイCarbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
- 配列類似性Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
- 翻訳後修飾S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
ISGylated. - 細胞内局在Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
- Information by UniProt
-
参照データベース
- Entrez Gene: 2597 Human
- Entrez Gene: 100042025 Mouse
- Entrez Gene: 14433 Mouse
- Entrez Gene: 24383 Rat
- Entrez Gene: 685186 Rat
- Omim: 138400 Human
- SwissProt: P04406 Human
- SwissProt: P16858 Mouse
see all -
別名
- 38 kDa BFA-dependent ADP-ribosylation substrate antibody
- aging associated gene 9 protein antibody
- Aging-associated gene 9 protein antibody
see all
Anti-GAPDH antibody 画像
-
Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody (ab83956)ICC/IF image of ab83956 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab83956 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- chicken (ab96947) IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
-
Western blot - GAPDH antibody (ab83956)Anti-GAPDH antibody (ab83956) at 1 µg/ml + HeLa Cell Lysate at 15 µg
Predicted band size : 36 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Anti-GAPDH antibody (ab83956) 使用論文
ab83956 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"