製品の概要

  • 製品名
    Anti-GAPDH antibody
    GAPDH 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to GAPDH
  • 由来種
    Chicken
  • アプリケーション
    適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A 16 amino acid synthetic peptide from near the carboxy-terminus of Human GAPDH (Swiss-Prot P04406).

  • ポジティブ･コントロール
    • HeLa Cell Lysate. This antibody gave a positive result when used in the following formaldehyde fixed cell lines: HeLa

アプリケーション

Our Abpromise guarantee covers the use of ab83956 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 36 kDa.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 5 µg/ml.

ターゲット情報

  • 機能
    Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
  • パスウェイ
    Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 1/5.
  • 配列類似性
    Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.
  • 翻訳後修飾
    S-nitrosylation of Cys-152 leads to interaction with SIAH1, followed by translocation to the nucleus.
    ISGylated.
  • 細胞内局在
    Cytoplasm > cytosol. Nucleus. Cytoplasm > perinuclear region. Membrane. Translocates to the nucleus following S-nitrosylation and interaction with SIAH1, which contains a nuclear localization signal (By similarity). Postnuclear and Perinuclear regions.
  • Information by UniProt
  • 参照データベース
    see all
  • 別名
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    • EC 1.2.1.12 antibody
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    • G3P_HUMAN antibody
    • G3PD antibody
    • G3PDH antibody
    • GAPD antibody
    • GAPDH antibody
    • Glyceraldehyde 3 phosphate dehydrogenase antibody
    • Glyceraldehyde-3-phosphate dehydrogenase antibody
    • HEL-S-162eP antibody
    • KNC-NDS6 antibody
    • MGC102544 antibody
    • MGC102546 antibody
    • MGC103190 antibody
    • MGC103191 antibody
    • MGC105239 antibody
    • MGC127711 antibody
    • MGC88685 antibody
    • OCAS, p38 component antibody
    • OCT1 coactivator in S phase, 38-KD component antibody
    • peptidyl cysteine S nitrosylase GAPDH antibody
    • Peptidyl-cysteine S-nitrosylase GAPDH antibody
    • wu:fb33a10 antibody
    see all

画像

  • Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody (ab83956)
    Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody (ab83956)

    ICC/IF image of ab83956 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab83956 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- chicken (ab96947) IgY (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • Western blot - GAPDH antibody (ab83956)
    Western blot - GAPDH antibody (ab83956)
    Anti-GAPDH antibody (ab83956) at 1 µg/ml + HeLa Cell Lysate at 15 µg

    Predicted band size: 36 kDa
    Observed band size: 42 kDa (why is the actual band size different from the predicted?)

参考文献

This product has been referenced in:
  • Osório C  et al. Selective regulation of axonal growth from developing hippocampal neurons by tumor necrosis factor superfamily member APRIL. Mol Cell Neurosci 59:24-36 (2014). WB ; Mouse . Read more (PubMed: 24444792) »
  • McCoy DD  et al. Enhanced insulin clearance in mice lacking TRPM8 channels. Am J Physiol Endocrinol Metab 305:E78-88 (2013). Read more (PubMed: 23651844) »

See all 2 Publications for this product

ab83956 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

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